Spatiotemporal filtering in calcium control of mitochondrial function

钙控制线粒体功能的时空过滤

• 批准号: BB/D013852/1
• 负责人: Julian Dow
• 金额: $ 39.5万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD013852%2F1
• 关键词: Spatiotemporal; filtering; calcium; control; mitochondrial

Calcium plays a central role in all cells and tissues, so the monitoring of cellular calcium is of major importance in both life sciences research and drug development. The critical contribution of cell organelles (mitochondria or nucleus, for example) to the health of the cell has led to the development of methods in which calcium signals in these organelles can be measured. To this end, we have successfully developed calcium indicators (reporters) to track calcium events in organelles in living organisms (the fruitfly, Drosophila melanogaster). We have made the first fruitflies which express calcium reporters in the mitochondria. The mitochondria are powerhouses which generate energy for the cell, and calcium signals are an important part of this process. Malfunction of mitochondrial calcium has been implicated in many disease processes and as most cells have distinct populations of mitochondria, determining the specific function of these in intact tissue would be novel and useful. At a cellular level, fruitflies and humans are very similar; so the fruitfly is an excellent model in which to study mitochondrial calcium signalling in an intact tissue or animal. These studies will allow us to find out how calcium signalling processes in this important organelle affects tissue function in a non-disease situation.

钙在所有细胞和组织中起着核心作用，因此细胞钙的监测在生命科学研究和药物开发中都具有重要意义。细胞器（例如线粒体或细胞核）对细胞健康的关键贡献导致了可以测量这些细胞器中钙信号的方法的发展。为此，我们已经成功地开发了钙指示剂（记者），以跟踪钙事件的细胞器中的活生物体（果蝇，果蝇）。我们已经制造了第一种在线粒体中表达钙报告基因的果蝇。线粒体是为细胞产生能量的发电站，钙信号是这一过程的重要组成部分。线粒体钙功能障碍与许多疾病过程有关，并且由于大多数细胞具有不同的线粒体群体，因此确定完整组织中这些线粒体的特定功能将是新颖且有用的。在细胞水平上，果蝇和人类非常相似;因此果蝇是研究完整组织或动物中线粒体钙信号的绝佳模型。这些研究将使我们能够了解在非疾病情况下，这个重要细胞器中的钙信号传导过程如何影响组织功能。

项目成果

Data-mining the FlyAtlas online resource to identify core functional motifs across transporting epithelia.

• DOI: 10.1186/1471-2164-14-518
• 发表时间: 2013-07-30
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Chintapalli VR;Wang J;Herzyk P;Davies SA;Dow JA
• 通讯作者: Dow JA

Mislocalization of mitochondria and compromised renal function and oxidative stress resistance in Drosophila SesB mutants.

• DOI: 10.1152/physiolgenomics.00147.2009
• 发表时间: 2010-03
• 期刊: Physiological genomics
• 影响因子: 4.6
• 作者: Selim Terhzaz;P. Cabrero;Venkateswara R. Chintapalli;S. Davies;J. Dow

A Drosophila systems approach to xenobiotic metabolism.

果蝇系统的外源代谢方法。

• DOI: 10.1152/physiolgenomics.00018.2007
• 发表时间: 2007
• 期刊: Physiological genomics
• 影响因子: 4.6
• 作者: Yang J

A comprehensive transcriptomic view of renal function in the malaria vector, Anopheles gambiae.

• DOI: 10.1016/j.ibmb.2015.05.007
• 发表时间: 2015-12
• 期刊: Insect biochemistry and molecular biology
• 影响因子: 3.8
• 作者: G. Overend;P. Cabrero;K. Halberg;L. Ranford-Cartwright;D. Woods;S. Davies;J. Dow