FlyAtlas: a comprehensive online atlas of gene expression in Drosophila

FlyAtlas：果蝇基因表达的综合在线图谱

• 批准号: BB/F010397/1
• 负责人: Julian Dow
• 金额: $ 34.54万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF010397%2F1
• 关键词: FlyAtlas; comprehensive; online; atlas; gene

Multicellular organisms, from humans to flies to plants, have distinct tissues specialized for distinct tasks. What makes one tissue different from another? Every cell in the body contains the same genetic code (of 15000-25000 genes, depending on the organism); but different tissues switch on (express) different subsets of these genes. For example, skin is skin because it expresses keratin, and liver is liver because it expresses detoxifying enzymes. To understand how organisms work, therefore, we need to know which genes are expressed in which tissues. There are many techniques for mapping gene expression levels, but most are laborious and prone to error. By contrast, DNA microarrays allow the relatively precise measurement of expression levels of all genes in a genome in a SINGLE experiment. A comprehensive, microarray-based 'atlas' of gene expression in specific tissues and cell types thoughout an organism is thus a fantastically useful resource to a whole community; if the job is done properly, there is no need to do it again. This proposal is to extend such a service for the Drosophila (fruit fly) research community, both in the UK and worldwide. A pilot project (with 9 adult tissues) has attracted over two thousand users in its first 6 months, confirming the need for such a resource. We propose to extend the resource to further tissues and further life stages, with the data easily accessible on the web (at flyatlas.org). The resource will allow scientists to target their effort more precisely, saving money and time, and reducing unnecessary animal use.

多细胞生物，从人类到苍蝇到植物，都有不同的组织，专门负责不同的任务。是什么使一个组织与另一个组织不同？体内的每个细胞都含有相同的遗传密码（根据生物体的不同，有15000-25000个基因）；但是不同的组织开启（表达）这些基因的不同子集。例如，皮肤之所以是皮肤，是因为它表达角蛋白，肝脏之所以是肝脏，是因为它表达解毒酶。因此，为了了解生物体是如何工作的，我们需要知道哪些基因在哪些组织中表达。绘制基因表达水平的技术有很多，但大多数都很费力，而且容易出错。相比之下，DNA微阵列可以在一次实验中相对精确地测量基因组中所有基因的表达水平。因此，一个全面的、基于微阵列的基因表达图谱在整个生物体的特定组织和细胞类型中是一个非常有用的资源。如果工作做得很好，就没有必要再做一次。这项提议是为了将这项服务扩展到英国和全世界的果蝇研究界。一个试点项目（有9个成人组织）在头6个月吸引了2000多名用户，证实了对这种资源的需求。我们建议将资源扩展到更多的组织和生命阶段，这些数据可以在网上轻松访问（flyatlas.org）。该资源将使科学家能够更精确地定位他们的工作目标，节省金钱和时间，并减少不必要的动物使用。

项目成果

Salty dog, an SLC5 symporter, modulates Drosophila response to salt stress.

• DOI: 10.1152/physiolgenomics.90360.2008
• 发表时间: 2009-03
• 期刊: Physiological genomics
• 影响因子: 4.6
• 作者: K. Stergiopoulos;P. Cabrero;S. Davies;J. Dow

Functional correlates of positional and gender-specific renal asymmetry in Drosophila.

• DOI: 10.1371/journal.pone.0032577
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Chintapalli VR;Terhzaz S;Wang J;Al Bratty M;Watson DG;Herzyk P;Davies SA;Dow JA
• 通讯作者: Dow JA