PARASITE REGULATORY MEDIATORS AND THEIR TARGET SITES

寄生虫调节介质及其靶位点

• 批准号: 3284306
• 负责人: DAVY JONES
• 金额: $ 11.7万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284306
• 关键词: Hymenoptera; affinity labeling; arthropod nonpollutant control; arthropod poison; bioassay; ecdysone; electron microscopy; endocrine transplantation; gel electrophoresis; high performance liquid chromatography; hormone regulation /control mechanism; host organism interaction; insect virus; juvenile hormones; larva; metamorphosis; molting; radioimmunoassay; virus DNA

The objective of the proposed research is to use the Trichoplusia ni-Chelonus spp. system to develop a basic understanding of arthropod development and how parasites (and man) may manipulate the host now been shown to induce several forms Chelonus parasites have now been shown to induce several forms of redirected development of their insect hosts by mechanisms that had not heretofore been considered or realized as possible. Thus, we seek an understanding of the components of the manipulated pathways and how these are manipulated by the parasite. The specific aims of the proposed research are 1) Identify the specific biochemical components of the female wasp which are responsible for redirected host development, 2) Purify the active factor(s) and 3) Identify of the target site of the factor(s) in the host. The methodology for identifying the components of the wasp which redirect development involved the use of several different approaches, each of which addresses the question from a different direction. The venom material, and viral and non- viral fractions of the calyx fluid, will be separately bioassayed by injection into host eggs. The eggs will be observed for redirected development. Also, antibodies active against each of these three materials will be injected into previously injected or naturally stung eggs so as to inhibit the action of the material. The methodology for the purifying the active factor(s) will involve both classical purification techniques (gradients, column chromatography, electrophoretic procedures, etc.) and the use of affinity columns based on antibodies. The methodology for the identification of the target site will involve injection of purified factor(s) radiolabelled by natural or organic chemistry means to follow their binding to the putative target. Alternatively, photaffinity procedures will also be used. As a third alternative, antibodies will used in immunohistochemical efforts to identify the targets site.

拟议研究的目的是利用 Trichoplusia 镍龟属系统的基本了解 节肢动物的发育以及寄生虫（和人类）如何 现在已证明操纵宿主可诱导多种形式 龟寄生虫现已被证明可诱导多种形式 通过机制重新定向昆虫宿主的发育 迄今为止，这一点尚未被考虑或实现。 因此，我们寻求对组成部分的理解 被操纵的途径以及这些途径是如何被操纵的 寄生虫。 拟议研究的具体目标是 1) 鉴定雌性黄蜂的特定生化成分 负责重定向主机开发，2) Purify 活性因子和 3) 目标位点的识别 主机中的因素。 识别黄蜂成分的方法 重定向开发涉及使用几个 不同的方法，每种方法都解决来自 不同的方向。 毒液物质，病毒性和非 萼液的病毒部分将单独进行生物测定 通过注射到宿主卵中。 鸡蛋将被观察 重定向发展。 此外，抗体对每种都有活性 这三种材料将被注入到先前注入或 自然蜇卵，从而抑制物质的作用。 纯化活性因子的方法将 涉及两种经典纯化技术（梯度、柱 色谱法、电泳程序等）和使用 基于抗体的亲和柱。 方法论 目标位点的识别将涉及注射纯化的 通过天然或有机化学手段放射性标记的因子 跟踪它们与假定目标的结合。 或者， 还将使用光亲和程序。 作为第三种选择， 抗体将用于免疫组织化学工作来识别 目标站点。