PARASITE REGULATORY MEDIATORS AND THEIR TARGET SITES

寄生虫调节介质及其靶位点

• 批准号: 3284302
• 负责人: DAVY JONES
• 金额: $ 1.7万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284302
• 关键词: Hymenoptera; affinity labeling; arthropod nonpollutant control; arthropod poison; bioassay; ecdysone; electron microscopy; endocrine transplantation; gel electrophoresis; high performance liquid chromatography; hormone regulation /control mechanism; host organism interaction; insect virus; juvenile hormones; larva; metamorphosis; molting; radioimmunoassay; virus DNA

The objective of the proposed research is to use the Trichoplusia ni-Chelonus spp. system to develop a basic understanding of arthropod development and how parasites (and man) may manipulate the host now been shown to induce several forms Chelonus parasites have now been shown to induce several forms of redirected development of their insect hosts by mechanisms that had not heretofore been considered or realized as possible. Thus, we seek an understanding of the components of the manipulated pathways and how these are manipulated by the parasite. The specific aims of the proposed research are 1) Identify the specific biochemical components of the female wasp which are responsible for redirected host development, 2) Purify the active factor(s) and 3) Identify of the target site of the factor(s) in the host. The methodology for identifying the components of the wasp which redirect development involved the use of several different approaches, each of which addresses the question from a different direction. The venom material, and viral and non- viral fractions of the calyx fluid, will be separately bioassayed by injection into host eggs. The eggs will be observed for redirected development. Also, antibodies active against each of these three materials will be injected into previously injected or naturally stung eggs so as to inhibit the action of the material. The methodology for the purifying the active factor(s) will involve both classical purification techniques (gradients, column chromatography, electrophoretic procedures, etc.) and the use of affinity columns based on antibodies. The methodology for the identification of the target site will involve injection of purified factor(s) radiolabelled by natural or organic chemistry means to follow their binding to the putative target. Alternatively, photaffinity procedures will also be used. As a third alternative, antibodies will used in immunohistochemical efforts to identify the targets site.

这项研究的目的是利用粉纹夜蛾 龟属系统开发的基本理解 节肢动物的发展以及寄生虫（和人）如何 操纵宿主现在被证明可以诱导几种形式的 海龟寄生虫现在已经被证明可以诱导几种形式的 它们的昆虫宿主通过 这是迄今为止还没有被认为是可能的。 因此，我们寻求理解的组成部分， 操纵的途径以及这些途径是如何被 寄生虫 拟议研究的具体目标是：1） 识别出雌蜂的特定生化成分 负责重定向主机开发，2）Purify 活性因子和3）确定的目标网站的 主机中的因素。 识别黄蜂成分的方法 这涉及到使用几个 不同的方法，其中每一个解决问题，从 一个不同的方向 毒液物质，病毒和非病毒- 将对花萼液的病毒成分进行单独的生物测定 注射到宿主卵中。 鸡蛋将被观察， 发展方向。 此外，抗体活性，针对每一个 这三种材料将被注入到先前注入的或 自然蛰卵，以抑制该物质的作用。 纯化活性因子的方法将 包括经典纯化技术（梯度，柱 色谱法、电泳程序等）和使用 基于抗体的亲和柱。 表方法 靶位点的鉴定将涉及注射纯化的 通过天然或有机化学手段进行放射性标记的因子， 与假定的目标结合。 可选择地， 还将使用光亲和性方法。 作为第三种选择， 抗体将用于免疫组织化学工作， 目标网站。