PARASITE MANIPULATION OF HOST INSECT ENDOCRINOLOGY

寄主昆虫内分泌学的寄生虫操纵

• 批准号: 3284304
• 负责人: DAVY JONES
• 金额: $ 9.1万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284304
• 关键词: arthropod nonpollutant control; bioassay; ecdysone; electron microscopy; endocrine transplantation; gel electrophoresis; high performance liquid chromatography; hormone regulation /control mechanism; host organism interaction; insect virus; juvenile hormones; larva; metamorphosis; molting; radioimmunoassay; virus DNA

Our objective is to use parasite induced precocious host metamorphosis as a novel model system for probing developmental regulation. Parasitic wasps (Chelonus spp.) induce their hosts to precociously initiate the developmental program for metamorphosis through a mechanism of host regulation undescribed for either vertebrate or invertebrate parasites. In addition, the premature transition to the adult is subsequently blocked midway through. Further, the internal parasite need not be, and often is not, present when the effects of host regulation take place. The specific aims of the proposal are: A) Determine the basis for initiation and completion of metamorphosis in Trichoplusia ni. This aim will be accomplished by study of critical growth thresholds, changes in hormone titers and bioassays of tissue commitment during larval development. B) Delineate the mechanism by which Chelonus spp. induce precocious host metamorphosis, by examining changes in growth thresholds, hormone titers and tissue comitment in host larvae. C) Define the endocrine basis of cessation of metamorphosis during the premature prepupal stage. This effect of host regulation will be investigated by hormone titering, hormonal treatments parabiosis and implantation of active endocrine glands. D) Determine now these effects occurs even in the absence of the internal parasite and what is the causative agent(s) from the parasite. Various treatments to remove internal parasites or denature parasite secretory factors will show the source and nature of the agent(s).

我们的目的是利用寄生虫诱导的早熟宿主变态作为一种 探索发育规律的新模型系统。 寄生蜂 （Chelonus spp.）诱导宿主提前启动 通过宿主机制变态的发育程序 对脊椎动物或无脊椎动物寄生虫的调节未描述。 在 此外，过早过渡到成年的过程随后会被阻止 中途。 此外，内部寄生虫不需要，并且通常是 不存在，当宿主调节的影响发生时。 具体 该提案的目标是：A）确定启动的基础， 粉纹夜蛾的变态完成。 这一目标将是 通过研究临界生长阈值，激素变化， 幼虫发育期间组织定型的滴度和生物测定。 （B） 描述了海龟属的机制。诱导早熟寄主 变态，通过检查生长阈值，激素滴度 和组织共生。 C.确定内分泌基础 在未成熟的预蛹阶段停止变态。 这 宿主调节作用将通过激素滴定来研究， 激素治疗联体共生和植入活性内分泌 腺体 D）现在确定这些影响即使在没有 内部寄生虫和什么是病原体（S）从寄生虫。 各种治疗，以消除内部寄生虫或变性寄生虫 分泌因子将显示试剂的来源和性质。