PARASITE MANIPULATION OF HOST INSECT ENDOCRINOLOGY

寄主昆虫内分泌学的寄生虫操纵

• 批准号: 3284304
• 负责人: DAVY JONES
• 金额: $ 9.1万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284304
• 关键词: arthropod nonpollutant control; bioassay; ecdysone; electron microscopy; endocrine transplantation; gel electrophoresis; high performance liquid chromatography; hormone regulation /control mechanism; host organism interaction; insect virus; juvenile hormones; larva; metamorphosis; molting; radioimmunoassay; virus DNA

Our objective is to use parasite induced precocious host metamorphosis as a novel model system for probing developmental regulation. Parasitic wasps (Chelonus spp.) induce their hosts to precociously initiate the developmental program for metamorphosis through a mechanism of host regulation undescribed for either vertebrate or invertebrate parasites. In addition, the premature transition to the adult is subsequently blocked midway through. Further, the internal parasite need not be, and often is not, present when the effects of host regulation take place. The specific aims of the proposal are: A) Determine the basis for initiation and completion of metamorphosis in Trichoplusia ni. This aim will be accomplished by study of critical growth thresholds, changes in hormone titers and bioassays of tissue commitment during larval development. B) Delineate the mechanism by which Chelonus spp. induce precocious host metamorphosis, by examining changes in growth thresholds, hormone titers and tissue comitment in host larvae. C) Define the endocrine basis of cessation of metamorphosis during the premature prepupal stage. This effect of host regulation will be investigated by hormone titering, hormonal treatments parabiosis and implantation of active endocrine glands. D) Determine now these effects occurs even in the absence of the internal parasite and what is the causative agent(s) from the parasite. Various treatments to remove internal parasites or denature parasite secretory factors will show the source and nature of the agent(s).

我们的目标是利用寄生虫诱导的早熟宿主变态作为一种 探索发展规律的新模式体系。寄生蜂 (Cheronus spp.)诱导它们的宿主早熟地启动 通过寄主机制进行变态的发育程序 对于脊椎动物或无脊椎动物寄生虫的调节未被描述。在……里面 此外，过早过渡到成人的过程随后被阻断。 中途。此外，内部寄生虫不需要，而且通常是 不是，而是在寄主监管的影响发生时出现。具体的 该提案的目的是：a)确定发起的基础和 杜氏毛霉完成变态。这一目标将是 通过研究临界生长阈值、激素变化来完成 幼虫发育过程中组织承诺的滴度和生物测定。b) 阐明了贝壳藻的致病机制。诱导早熟宿主 变态，通过检测生长阈值、激素效价的变化 和寄主幼虫的组织伴生。C)确定以下物质的内分泌基础 在早熟的预蛹阶段停止变态。这 寄主调节的效果将通过激素滴定来调查， 激素治疗异育和活性内分泌植入 腺体。D)现在确定这些影响即使在没有 内部寄生虫以及来自寄生虫的病原体(S)是什么。 清除体内寄生虫或变性寄生虫的各种治疗方法 分泌因素将显示代理人的来源和性质(S)。