DIPTERAN DEVELOPMENT REGULATED BY A CELL DEATH INHIBITOR

细胞死亡抑制剂调控双翅目发育

• 批准号: 2906991
• 负责人: DAVY JONES
• 金额: $ 19.89万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-13 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2906991
• 关键词: Anopheles; Drosophilidae; apoptosis; binding proteins; chimeric proteins; gene expression; genetic mapping; genetically modified animals; growth /development; immunofluorescence technique; immunoprecipitation; in situ hybridization; inhibitor /antagonist; northern blottings; point mutation; protein protein interaction; protein structure function; transcription factor; western blottings; yeast two hybrid system

DESCRIPTION: (Adapted from the Applicant's Abstract): The long-range goal of this research is to determine the cellular and developmental impact of an unusually structured inhibitor of apoptosis which has recently been discovered in Drosophila. Apoptosis and its precisely controlled inhibition are fundamental to the proper formation and development of the dipteran embryo, larva and adult. This new insect apoptosis inhibitor, deterin, occurs in the embryo, larva and reproductive adult female. It has been shown to block apoptosis induced by reaper (an insect-specific apoptosis activator) in both transgenic flies and cultured cells. The impact of deterin on insectan developmental processes and apoptotic regulators can only be determined in an insect system. This will be done by addressing three specific aims. 1.) The precise temporal and tissue specific expression of the deterin transcript and its encoded protein will be determined in developmental studies. 2.) Hypotheses about the developmental functions and antiapoptotic actions of drosophilan and mosquito deterin during embryonic, metamorphic and reproductive development and in normal and apoptotic cells will be tested. 3.) The molecular mechanism of action of the deterin protein, including its target sites will be assessed.

描述：（根据申请人的摘要改编）： 这项研究是为了确定一个细胞和发育影响 凋亡的异常结构化抑制剂，最近被发现 在果蝇中。凋亡及其精确控制的抑制是 是双翅目胚胎的适当形成和发展的基础， 幼虫和成人。这种新的昆虫凋亡抑制剂Deterin发生在 胚胎，幼虫和生殖成年女性。已显示它可以阻止 被收割机（一种昆虫特异性凋亡激活剂）诱导的凋亡 转基因苍蝇和培养细胞。 Deterin对昆虫的影响 发展过程和凋亡调节剂只能在 昆虫系统。这将通过解决三个具体目标来完成。 1.） Deterin转录本的精确时间和组织特异性表达和 它的编码蛋白将在发育研究中确定。 2.）假设 关于果蝇和 胚胎，变质和生殖发育期间的蚊子去蛋白 在正常和凋亡细胞中，将测试。 3.）分子机制 将评估定质蛋白的作用，包括其靶位点。