ISOLATION OF COMPOUNDS PROMOTING CELL DIVISION IN PLANTS

我们重新调查了之前的一份报告，其中提到了几部小说的存在 冠瘿瘤中的细胞分裂促进化合物。 水滴的使用 逆流色谱和 5Mu 反相 HPLC 已允许 六种结构异构体的绝对纯化。 这些化合物通过抑制促进细胞分裂的活性 cAMP依赖性蛋白激酶为生长和发育提供了新的视角 高等植物细胞的分裂。 了解角色的第一步 这些细胞分裂因子的完整结构分配。 现已开发出光谱方法，以便完整地 可以进行结构分配。 这些研究将有助于了解细胞生长和分裂 分子水平上的高等植物，可以进行比较 介于正常状态和肿瘤状态之间。

We have reinvestigated an earlier report of the presence of several novel cell-division promoting compounds in crown gall tumors. The use of droplet counter-current chromatography and 5Mu reverse phase HPLC has allowed for absolute purification of six structural isomers. The activity of these compounds in promoting cell division by inhibiting cAMP dependent protein kinases provides a new perspective on the growth and division in higher plant cells. The first step in understanding the role of these factors in cell division is their complete structural assignment. Spectroscopic methodologies have now been developed so that complete structure assignment can be made. These studies will permit an understanding of cell growth and division in higher plants at a molecular level and will enable a comparison to be made between the normal and tumorous states.