CHEMICAL & GENETIC APPROACHES TO DEFINE CELL ACTIVATION

该项目旨在融合两个实验室的专业知识 不同的机构，以贡献人类对细胞的理解 成长和发展。这些流程定义了体系结构和运行状况 从人类到高等植物的所有真核生物。植物细胞， 然而，为所有真核生长和 发展，一种现在可以在化学和基因上操纵的发展， 甚至到了转基因生物的程度。 具体地说，控制独特生长的生物合成的酶 将分离出脱氢二苯甲醇葡萄糖苷(DCG)， 并克隆了它们各自的基因。各种化学品和 基因方法将被用来操纵这些基因的生产 体内生长因子对细胞因子水平和活性的影响 酵素。对一条路径的操纵应该简化另一条路径 肯定参与细胞控制的相互作用的系统 成长。DCG产生途径的定义和研究进展 对所涉及的生物催化剂的控制应该是 理解生物化学中的一个黑匣子--信息是如何 在控制细胞生长的过程中接收和处理。

This project is designed to fuse the expertise of laboratories at two different institutions in order to contribute man's understanding of cell growth and development. These processes define the architecture and health of all eucaryotic organisms from man to higher plants. Plant cells, however, provide a simplified model system for all eucaryotic growth and development, one that can now be chemically and genetically manipulated, even to the point of transgenic organisms. Specifically, the enzymes controlling the biosynthesis of a unique growth factor, the dehydrodiconiferyl alcohol glucosides (DCG), will be isolated, characterized, and their respective genes cloned. Various chemical and genetic approaches will be employed to manipulate the production of these growth factors in vivo manipulating the levels and activities of the enzymes. The manipulation of one pathway should simplify the other interacting systems that are certainly involved in the control of cell growth. The definition of the pathway involved in DCG production and the control of the biocatalysts involved should provide the first step in understanding of one of the black boxes of biochemistry-how information is received and processed in the control of cell growth.