CHEMICAL & GENETIC APPROACHES TO DEFINE CELL ACTIVATION

该项目旨在融合两个实验室的专业知识， 不同的机构，以促进人类对细胞的理解， 增长和发展。 这些过程定义了体系结构和运行状况 从人类到高等植物的所有真核生物。 植物细胞， 然而，提供了用于所有真核生长简化模型系统， 一个现在可以通过化学和基因操纵的发展， 甚至到了转基因生物的地步。 具体来说，控制一种独特生长的生物合成的酶 将分离因子，脱氢双子叶醇葡糖苷（DCG）， 其特征在于，它们各自的基因被克隆。 各种化学和 将采用遗传方法来操纵这些基因的产生。 生长因子在体内操纵的水平和活动， 内切酶 对其中一条途径的操作应该会简化另一条途径 这些相互作用的系统肯定参与了细胞的控制， 增长 参与DCG产生的途径的定义和 控制所涉及的生物催化剂应该提供第一步， 了解生物化学的黑匣子之一-信息是如何 在细胞生长的控制下接收和处理。

This project is designed to fuse the expertise of laboratories at two different institutions in order to contribute man's understanding of cell growth and development. These processes define the architecture and health of all eucaryotic organisms from man to higher plants. Plant cells, however, provide a simplified model system for all eucaryotic growth and development, one that can now be chemically and genetically manipulated, even to the point of transgenic organisms. Specifically, the enzymes controlling the biosynthesis of a unique growth factor, the dehydrodiconiferyl alcohol glucosides (DCG), will be isolated, characterized, and their respective genes cloned. Various chemical and genetic approaches will be employed to manipulate the production of these growth factors in vivo manipulating the levels and activities of the enzymes. The manipulation of one pathway should simplify the other interacting systems that are certainly involved in the control of cell growth. The definition of the pathway involved in DCG production and the control of the biocatalysts involved should provide the first step in understanding of one of the black boxes of biochemistry-how information is received and processed in the control of cell growth.