Chemical & Genetic Approaches to Define Cell Activation

描述（由申请人提供）：出现了复杂的策略， 调节所有病原体中关键的营养/寄生转变， 在许多情况下，这种监管是由两个组成部分的监管制度提供的。 植物病原体根癌农杆菌（Agrobacterium tumefaciens）的VirA/VirG系统是植物病原体根癌农杆菌（Agrobacterium tumefaciens）的VirA/VirG系统。 多个不同外部信号输入的临界积分器模型 对发病机制的承诺是必要的。它是唯一一个 输入信号是已知的，并且存在用于信号感知的模型。此外，委员会认为， 操纵基因组的遗传和分子工具已经到位， 控制vir基因表达的因素容易且安全地控制 在病原体本身。为了抓住这个机会，我们提出了一个 实验计划，使我们能够测试信号的分子模型， 感知、整合和传递致病信号。的 方法利用广泛的化学、物理和遗传方法， 在两个不同的实验室利用现有的资源， 机构职能体系这些研究的结果将使我们能够 确定如何识别、集成和转换信号输入， 双组分响应调节器。此外，这种特殊的信号系统 调节唯一已知的用于王国间基因转移的天然载体， 了解这一机制已经并将继续扩大范围， 在生物技术中的应用。

DESCRIPTION (provided by applicant): Sophisticated strategies have emerged to regulate the critical vegetative / parasitic transition in all pathogens, and in many cases, this regulation is provided by two-component regulatory systems. The VirA/VirG system of the plant pathogen Agrobacterium tumefaciens is the model of a critical integrator of several different external signal inputs necessary for the commitment to pathogenesis. it is the only system for which the input signal is known and a model for signal perception exists. Moreover, the genetic and molecular tools to manipulate the genome are in place, allowing the factors that control vir gene expression to be easily and safely controlled in the pathogenetic organism itself. To seize this opportunity, we propose an experimental plan that allows us to test a molecular model for signal perception, integration, and transmission in pathogenetic signaling. The approach utilizes a wide range of chemical, physical, and genetic methods that exploit the resources available in different laboratories at two distinct institutions. The results of these studies will place us in the position to determine how signal input is recognized, integrated, and transduced in two-component response regulators. In addition, this specific signaling system regulates the only know natural vector for inter-Kingdom gene transfer and understanding this mechanism has already and will continue to widened the range of its use in biotechnology.