Chemical & Genetic Approaches to Define Cell Activation

描述(由申请者提供)：出现了复杂的策略 调控所有病原体的关键营养/寄生转变，以及 在许多情况下，这种监管是由两个组成部分的监管体系提供的。 植物病原菌根癌农杆菌的病毒/病毒系统是 几种不同外部信号输入的临界积分器模型 对于致病机制的承诺是必要的。这是唯一一个可以 输入信号是已知的，并且存在信号感知模型。此外， 操纵基因组的遗传和分子工具已经到位，允许 控制病毒基因表达的因素容易和安全地控制 在致病有机体本身。为了抓住这个机会，我们提出了一个 允许我们测试信号的分子模型的实验计划 致病信号中的感知、整合和传递。这个 该方法利用了广泛的化学、物理和遗传方法， 在两个不同的实验室开发可用的资源 机构。这些研究的结果将使我们能够 确定如何识别、集成和转换信号输入 双分量响应调节器。此外，这一特定的信号系统 调节唯一已知的跨王国基因转移的自然载体和 了解这一机制已经并将继续扩大范围 它在生物技术中的应用。

DESCRIPTION (provided by applicant): Sophisticated strategies have emerged to regulate the critical vegetative / parasitic transition in all pathogens, and in many cases, this regulation is provided by two-component regulatory systems. The VirA/VirG system of the plant pathogen Agrobacterium tumefaciens is the model of a critical integrator of several different external signal inputs necessary for the commitment to pathogenesis. it is the only system for which the input signal is known and a model for signal perception exists. Moreover, the genetic and molecular tools to manipulate the genome are in place, allowing the factors that control vir gene expression to be easily and safely controlled in the pathogenetic organism itself. To seize this opportunity, we propose an experimental plan that allows us to test a molecular model for signal perception, integration, and transmission in pathogenetic signaling. The approach utilizes a wide range of chemical, physical, and genetic methods that exploit the resources available in different laboratories at two distinct institutions. The results of these studies will place us in the position to determine how signal input is recognized, integrated, and transduced in two-component response regulators. In addition, this specific signaling system regulates the only know natural vector for inter-Kingdom gene transfer and understanding this mechanism has already and will continue to widened the range of its use in biotechnology.