IDRM Spatial Transcriptomics Platform

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Relationships between different resident and infiltrating cell types with the cellular matrix are recognized as key components that define tissues. One way to profile individual cells is to use technologies that allow all the genes in each cell to be sequenced so we can determine which genes might regulate how the cell behaves in a tissue of interest, either during health or disease. However, this involves separating out cells from the tissue and so we lose important information as to how cells interact with each other and the local environment. To this end, being able to profile genes within cells whilst they remain intact within tissues is a major advance, as has been pioneered commercially by NanoString with the GeoMx Spatial Profiler. We wish to utilise such a profiler within the Institute of Developmental Biology & Regenerative Medicine (IDRM) at the University of Oxford, and also make it available to colleagues in the Kennedy Institute of Inflammation and Rheumatology (KIR) and Institute of Biomedical Engineering (IBME) on the Old Road Medical Campus and across the wider Medical Sciences and Maths, Physics and Life Sciences Divisions. Specifically, we propose to use the GeoMx Spatial Profiler to provide molecular profiles of neighbouring cells within tissues, and to detect changes in gene or protein expression during tissue/organ development, normal function and in response to injury or disease.