IDRM Spatial Transcriptomics Platform

IDRM 空间转录组学平台

• 批准号: BB/W019612/1
• 负责人: Paul Riley
• 金额: $ 32.73万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW019612%2F1
• 关键词: IDRM; Spatial; Transcriptomics; Platform

Relationships between different resident and infiltrating cell types with the cellular matrix are recognized as key components that define tissues. One way to profile individual cells is to use technologies that allow all the genes in each cell to be sequenced so we can determine which genes might regulate how the cell behaves in a tissue of interest, either during health or disease. However, this involves separating out cells from the tissue and so we lose important information as to how cells interact with each other and the local environment. To this end, being able to profile genes within cells whilst they remain intact within tissues is a major advance, as has been pioneered commercially by NanoString with the GeoMx Spatial Profiler. We wish to utilise such a profiler within the Institute of Developmental Biology & Regenerative Medicine (IDRM) at the University of Oxford, and also make it available to colleagues in the Kennedy Institute of Inflammation and Rheumatology (KIR) and Institute of Biomedical Engineering (IBME) on the Old Road Medical Campus and across the wider Medical Sciences and Maths, Physics and Life Sciences Divisions. Specifically, we propose to use the GeoMx Spatial Profiler to provide molecular profiles of neighbouring cells within tissues, and to detect changes in gene or protein expression during tissue/organ development, normal function and in response to injury or disease.

不同的常驻和浸润细胞类型与细胞基质之间的关系被认为是定义组织的关键组成部分。分析单个细胞的一种方法是使用允许每个细胞中所有基因测序的技术，这样我们就可以确定哪些基因可能调节细胞在健康或疾病期间在感兴趣的组织中的行为。然而，这涉及将细胞从组织中分离出来，因此我们丢失了关于细胞如何相互作用以及局部环境的重要信息。为此，能够分析细胞内的基因，同时它们在组织内保持完整是一个重大的进步，正如NanoString与GeoMx Spatial Profiler在商业上开创的那样。我们希望在牛津大学发育生物学与再生医学研究所（IDRM）内利用这样的分析器，并将其提供给肯尼迪炎症和流变学研究所（KIR）和生物医学工程研究所（IBME）的同事，以及更广泛的医学科学和数学，物理学和生命科学部门。具体而言，我们建议使用GeoMx空间轮廓仪提供组织内相邻细胞的分子特征，并检测组织/器官发育、正常功能以及对损伤或疾病的反应中基因或蛋白质表达的变化。