MOLECULAR REGULATION OF IMMUNOGLOBULIN GENE EXPRESSION

在小鼠12号染色体上编码的基因是调节 免疫系统的发展。 这些包括免疫球蛋白重链 链基因本身，以及T细胞和至少一个 类肿瘤细胞。 这些基因已经被广泛研究，不仅 因为它们与免疫功能相关，但因为它们提供了 一个强大的模型系统，用于发育的分子遗传学分析， 在哺乳动物系统中。 在这里，我们建议使用以下组合： 重组DNA技术与体细胞遗传学， 定义小鼠12号染色体的其他遗传标记，并开始 确定这些基因的可能功能。 具体来说，重组DNA 技术将被用来克隆基因组DNA片段来自 染色体 用这些片段定义的DNA多态性将用于 扩展并填充染色体的孟德尔图和物理图。 滤膜杂交技术将用于鉴定这些克隆的DNA 在特定组织中以RNA水平表达的片段， 淋巴细胞发育的特定阶段。

Genes encoded on mouse chromosome 12 are central to the regulated development of the immune system. These include the immunoglobulin heavy chain genes themselves, as well as markers of T cells and at least one class of tumor cells. These genes have been widely studied, not only because of their relevance to immune function, but because they provide a powerful model system for the molecular genetic analysis of developmental processes in a mammalian system. Here, we propose to use a combination of recombinant DNA technology with somatic cell genetics to systematically define additional genetic markers of mouse chromosome 12, and to begin to define possible functions for these genes. Specifically, recombinant DNA techniques will be used to clone genomic DNA fragments derived from the chromosome. DNA polymorphisms defined with these fragments will be used to extend and fill in the Mendelian and physical maps of the chromosome. Filter hybridization techniques will be used to identify these cloned DNA fragments that are expressed at the RNA level in particular tissues and at particular stages in lymphocyte development.