ISOLATION OF COMPOUNDS PROMOTING CELL DIVISION IN PLANTS

我们重新调查了早先关于几种新的 细胞分裂促进化合物在冠瘿肿瘤。 水滴的使用 逆流色谱和5Mu反相HPLC允许 六种结构异构体的绝对纯化。 这些化合物通过抑制细胞分裂而促进细胞分裂的活性 cAMP依赖性蛋白激酶提供了一个新的视角， 高等植物细胞的分裂。 了解角色的第一步 这些因素在细胞分裂中的作用是它们完整的结构分配。 现在已经开发了光谱方法， 可以进行结构分配。 这些研究将使我们能够了解细胞的生长和分裂， 在分子水平上研究高等植物， 在正常和肿瘤状态之间。

We have reinvestigated an earlier report of the presence of several novel cell-division promoting compounds in crown gall tumors. The use of droplet counter-current chromatography and 5Mu reverse phase HPLC has allowed for absolute purification of six structural isomers. The activity of these compounds in promoting cell division by inhibiting cAMP dependent protein kinases provides a new perspective on the growth and division in higher plant cells. The first step in understanding the role of these factors in cell division is their complete structural assignment. Spectroscopic methodologies have now been developed so that complete structure assignment can be made. These studies will permit an understanding of cell growth and division in higher plants at a molecular level and will enable a comparison to be made between the normal and tumorous states.