An optical detector for latent fungal infection in produce

用于农产品中潜在真菌感染的光学检测器

基本信息

  • 批准号:
    BB/X003744/1
  • 负责人:
  • 金额:
    $ 23.52万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2023
  • 资助国家:
    英国
  • 起止时间:
    2023 至 无数据
  • 项目状态:
    未结题

项目摘要

Food waste is a major problem, with huge environmental and economic impact. A significant cause of waste are fungal infections. Ideally, fruit that has been infected should be removed from storage as soon as possible to prevent spread of the infection and potentially the loss of the entire store. However, fungal infections are often latent in their early stages, meaning they are inactive ion or just below the surface and cannot be detected at an early stage, e.g. before the fruit is stored. These latent infection can quickly progress during the storage, infection spreading to the neighbouring produce causing significant losses. If there was a reliable method to detect this latent infection, there is potential to significantly reduce wastage.High-resolution optical imaging methods have shown huge promise in medicine as a means of detecting disease, often at an early stage. There is therefore an obvious potential to translate this technology to agriculture. In particular, optical coherence tomography allows 3D images to be acquired in timescales of around a second, penetrating 1-2 mm into tissue. It provides structural information down to a resolution of less than 10 microns (0.01 mm) without the need for destructive sampling, staining or preparation of the sample in any way. However, while OCT has shown some promise for imaging plants and fruit for some applications, the simple structural information it provides may not provide reliable detection of the subtle changes associated with latent fungal infection.In this project we will investigate and demonstrate a new approach for early detection of fungal infection in fruits. We will bring together the expertise in the design and development of optical imaging systems at the Applied Optics Group, University of Kent, with the horticulture and plant pathology expertise of the Pest and Pathogen Ecology team at NIAB EMR. We will develop a new imaging device designed for the detection of latent, early stage fungus infection and test it using cherry samples known to be carrying the fungus Monilinia laxa.The physical principles of the device will be similar in its elementary structure to the conventional OCT, using a combination of a laser-scanning probe head, a broadband optical source and a fast spectrometer to allow fast 3D imaging. However, unlike in conventional OCT, which provides a static snapshot of the structure of the sample, new investigative modalities will be harnessed based on recent developments in the OCT field for biomedical diagnosis. These will be implemented to perform a more subtle analysis of fruit samples, based on acquiring multiple forms of functional or dynamic information, including looking at how the microstructure of the sample responds to an external mechanical stimulus, and measuring both the magnitude and the frequency of intrinsic fluctuations on the scale of cellular components. These three techniques, known as optical coherence elastography (OCE), speckle variance (SV), and the newly emerging dynamic OCT (DyOCT) will be implemented in a single device, allowing the fruit to be comprehensively evaluated.The sensitivity and accuracy of the optical tools (OCT, OCE, SV, DyOCT) will be benchmarked using destructive methods such as isolation of live pathogen, localisation using fluorescent microscopy and quantification of fungal biomass with quantitative PCR.
食物浪费是一个重大问题,对环境和经济造成巨大影响。废物的一个重要原因是真菌感染。理想情况下,应尽快将受感染的水果从储存中取出,以防止感染的传播和整个商店的潜在损失。然而,真菌感染通常在其早期阶段是潜伏的,这意味着它们是无活性的离子或仅在表面以下,并且在早期阶段(例如在水果储存之前)无法检测到。这些潜伏的感染可以在储存期间迅速发展,感染传播到邻近的产品,造成重大损失。如果有一种可靠的方法来检测这种潜伏感染,就有可能大大减少浪费。高分辨率光学成像方法在医学上显示出巨大的前景,作为一种检测疾病的手段,通常在早期阶段。因此,将这一技术应用于农业显然是有潜力的。特别是,光学相干断层扫描允许在大约一秒的时间尺度内采集3D图像,穿透1-2 mm进入组织。它提供的结构信息的分辨率小于10微米(0.01毫米),而不需要破坏性取样,染色或以任何方式制备样品。然而,尽管OCT在植物和水果的成像方面有一定的应用前景,但它提供的简单结构信息可能无法可靠地检测与潜在真菌感染相关的细微变化,在本项目中,我们将研究并展示一种新的方法,用于水果真菌感染的早期检测。我们将把肯特大学应用光学组在光学成像系统设计和开发方面的专业知识与NIAB EMR害虫和病原体生态学团队的园艺和植物病理学专业知识结合起来。我们将开发一种新的成像设备,用于检测潜伏的早期真菌感染,并使用已知携带真菌Monilinia拉克萨的樱桃样本进行测试。该设备的物理原理在基本结构上与传统OCT相似,使用激光扫描探头,宽带光源和快速光谱仪的组合,以实现快速3D成像。然而,与传统的OCT不同,它提供了样本结构的静态快照,新的研究模式将根据OCT领域的最新发展用于生物医学诊断。这些将被实施来执行水果样品的更微妙的分析,基于获取多种形式的功能或动态信息,包括查看样品的微观结构如何响应外部机械刺激,并测量细胞成分尺度上的内在波动的幅度和频率。这三种技术,被称为光学相干弹性成像(OCE)、散斑方差(SV),以及新出现的动态OCT(DyOCT),将在一台设备中实现,从而可以全面评估水果光学工具的灵敏度和准确度(OCT、OCE、SV、DyOCT)将使用破坏性方法进行基准测试,如分离活病原体,使用荧光显微镜进行定位,并使用定量PCR对真菌生物量进行定量。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Adrian Podoleanu其他文献

What Photonics Tools Can Do for Biosciences
光子学工具可以为生物科学做什么
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Adrian Podoleanu
  • 通讯作者:
    Adrian Podoleanu

Adrian Podoleanu的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Adrian Podoleanu', 18)}}的其他基金

Compact Forward-Viewing Endoscopic Optical Coherence Tomography
紧凑型前视内窥镜光学相干断层扫描
  • 批准号:
    EP/X000125/1
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Research Grant
5-Dimensional High-Resolution non-invasive assessment of mammalian Embryos (5DHiResE)
哺乳动物胚胎的 5 维高分辨率非侵入性评估 (5DHiResE)
  • 批准号:
    BB/S016643/1
  • 财政年份:
    2019
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Research Grant
REBOT: Robotic Endobronchial Optical Tomography
REBOT:机器人支气管内光学断层扫描
  • 批准号:
    EP/N019229/1
  • 财政年份:
    2016
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Research Grant
NOVEL METHOD FOR OPTICAL COHERENCE TOMOGRAPHY AND MULTIPLEXED SENSING
光学相干断层扫描和多重传感的新方法
  • 批准号:
    EP/H004963/1
  • 财政年份:
    2009
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Research Grant
Optical Coherence Tomography for Embryology
用于胚胎学的光学相干断层扫描
  • 批准号:
    BB/E002870/1
  • 财政年份:
    2006
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Research Grant

相似海外基金

Point-scanning confocal with area detector
点扫描共焦与区域检测器
  • 批准号:
    534092360
  • 财政年份:
    2024
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Major Research Instrumentation
Next Generation Water Cherenkov Detector Technology Development For The Study Of Supernova Neutrinos
用于超新星中微子研究的下一代水切伦科夫探测器技术开发
  • 批准号:
    MR/Y034082/1
  • 财政年份:
    2024
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Fellowship
Extending the Teach of Gravitational-Wave Detectors through Low Latency Data Products, Unmodeled Searches, and Detector Characterization
通过低延迟数据产品、未建模搜索和探测器表征扩展引力波探测器的教学
  • 批准号:
    2308862
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Standard Grant
Collaborative Research: RUI: Study of Nutron-Rich Nuclei and Neutron Detector Response
合作研究:RUI:富营养核和中子探测器响应的研究
  • 批准号:
    2311125
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Standard Grant
Collaborative Research: Equipment: MRI Consortium: Track 2 Development of a Next Generation Fast Neutron Detector
合作研究:设备:MRI 联盟:下一代快中子探测器的 Track 2 开发
  • 批准号:
    2320407
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Standard Grant
Collaborative Research: Equipment: MRI Consortium: Track 2 Development of a Next Generation Fast Neutron Detector
合作研究:设备:MRI 联盟:下一代快中子探测器的 Track 2 开发
  • 批准号:
    2320405
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Standard Grant
PFI–TT: Development of an Explainable and Robust Detector of Forged Multimedia and Cyber Threats using Artificial intelligence
PFI™TT:使用人工智能开发可解释且强大的伪造多媒体和网络威胁检测器
  • 批准号:
    2329858
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Continuing Grant
A new control scheme of the polarization-circulation speed-meter as a gravitational wave detector
一种新的引力波探测器偏振环流速度计控制方案
  • 批准号:
    23H01205
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Atom probe instrument for the imaging of hydrogen in materials – laser pulsing / detector upgrade
用于材料中氢成像的原子探针仪器 â 激光脉冲/探测器升级
  • 批准号:
    516600907
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Major Research Instrumentation
Development of dose measurement system for therapeutic carbon-ion beam using a glass block without quenching effect
开发使用无淬火效应的玻璃块的治疗碳离子束剂量测量系统
  • 批准号:
    23K14859
  • 财政年份:
    2023
  • 资助金额:
    $ 23.52万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了