ANALYSIS OF THE ANAEROBIC-STRESS RESPONSE IN MAIZE

我建议研究玉米的厌氧胁迫反应。厌氧 处理显著改变玉米的基因表达模式 苗木。在厌氧期间，会立即抑制 先有蛋白质合成，然后诱导基因表达 涉及合成大约20个厌氧专一性 蛋白质。在这些厌氧蛋白(ANP)中有酒精 脱氢酶(ADH)同工酶由Adh1和ADH2基因编码。 玉米的厌氧胁迫反应提供了一个独特的机会 描述一个由20个基因组成的家族的调控成分 都是协调表达的。此提案的总体目标是使用 强大的遗传学工具和重组DNA技术来分析这一点 高度受控的反应。我计划对这些基因进行鉴定和表征 参与植物对可能发生的环境胁迫的反应 在洪水期间在自然界中。这些基因包括剩余的 厌氧专一性基因和编码过渡多肽的基因 它们是在第一阶段优先合成的一组蛋白质 几个小时的厌氧处理。它还包括可能的交易 可能控制这种生理反应的基因。序列比较 并将进行表达研究，以确定 可能与转录诱导有关的厌氧基因 以及它们在厌氧过程中的选择性翻译。 预计这些研究将有助于 对真核基因作为一个功能单位的理解以及对某些 差异基因表达的各个方面。

I propose to study the anaerobic-stress response in Zea mays. Anaerobic treatment dramatically alters the pattern of gene expression in maize seedlings. During anaerobiosis there is an immediate repression of pre-existing protein synthesis followed by an induction of gene expression involving the synthesis of approximately twenty anaerobic-specific proteins. Among these anaerobic proteins (ANPs) are the alcohol dehydrogenase (ADH) isozymes encoded by the Adh1 and Adh2 genes. The anaerobic-stress response of maize offers a unique opportunity to characterize the regulatory components of a family of twenty genes which are coordinately expressed. The overall aim of this proposal is to use the powerful tools of genetics and recombinant DNA technology to analyze this highly regulated response. I plan to identify and characterize the genes involved in a plant's response to an environmental stress which would occur in nature during flooding. These genes include the remaining anaerobic-specific genes, and those encoding the transition polypeptides which are a group of proteins synthesized preferentially during the first few hours of anaerobic treatment. It also includes possible trans-acting genes that might control this physiological response. Sequence comparisons and expression studies will be carried out to identify regions within the anaerobic genes that may be responsible for the induction of transcription and their selective translation during anaerobiosis. It is anticipated that these studies will contribute toward the understanding of the eukaryotic gene as a functional unit and to some aspects of differential gene expression.