ANALYSIS OF THE ANAEROBIC-STRESS RESPONSE IN MAIZE

我建议研究玉米的厌氧应激反应。 厌氧 处理显著地改变了玉米中基因表达的模式 幼苗 在厌氧期间， 预先存在的蛋白质合成，随后诱导基因表达 包括合成大约20种厌氧特异性 proteins. 在这些厌氧蛋白质（ANP）中， 脱氢酶（ADH）同工酶由Adh 1和Adh 2基因编码。 玉米的厌氧应激反应提供了独特的机会， 描述了一个由20个基因组成的家族的调控成分， 是协调表达的。 本提案的总体目标是利用 遗传学和重组DNA技术的强大工具来分析这一点， 高度规范的反应。 我计划识别并描述 参与植物对环境压力的反应 在洪水期间。 这些基因包括剩余的 厌氧特异性基因和编码过渡多肽的基因 这是一组蛋白质，在第一次合成时优先合成， 几个小时的厌氧处理。 它还包括可能的反式作用 可能控制这种生理反应的基因 序列比较 和表达研究将进行，以确定区域内的 厌氧基因，可能是负责诱导转录 以及它们在厌氧期间的选择性翻译。 预计这些研究将有助于 了解真核基因作为一个功能单位， 差异基因表达方面。

I propose to study the anaerobic-stress response in Zea mays. Anaerobic treatment dramatically alters the pattern of gene expression in maize seedlings. During anaerobiosis there is an immediate repression of pre-existing protein synthesis followed by an induction of gene expression involving the synthesis of approximately twenty anaerobic-specific proteins. Among these anaerobic proteins (ANPs) are the alcohol dehydrogenase (ADH) isozymes encoded by the Adh1 and Adh2 genes. The anaerobic-stress response of maize offers a unique opportunity to characterize the regulatory components of a family of twenty genes which are coordinately expressed. The overall aim of this proposal is to use the powerful tools of genetics and recombinant DNA technology to analyze this highly regulated response. I plan to identify and characterize the genes involved in a plant's response to an environmental stress which would occur in nature during flooding. These genes include the remaining anaerobic-specific genes, and those encoding the transition polypeptides which are a group of proteins synthesized preferentially during the first few hours of anaerobic treatment. It also includes possible trans-acting genes that might control this physiological response. Sequence comparisons and expression studies will be carried out to identify regions within the anaerobic genes that may be responsible for the induction of transcription and their selective translation during anaerobiosis. It is anticipated that these studies will contribute toward the understanding of the eukaryotic gene as a functional unit and to some aspects of differential gene expression.