STRUCTURE OF DENITRIFICATION ENZYMES

反硝化酶的结构

• 批准号: 3289891
• 负责人: RUSSELL TIMKOVICH
• 金额: $ 7.83万
• 依托单位: UNIVERSITY OF ALABAMA IN TUSCALOOSA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3289891
• 关键词: Pseudomonas; bacteria; cytochrome c; electron transport; enzyme mechanism; enzyme structure; enzyme substrate analog; heme; hemoprotein structure; infrared spectrometry; mass spectrometry; nitrites; nuclear magnetic resonance spectroscopy; oxidoreductase; porphyrin biosynthesis; reduction

The proposed project will determine structural elements for the enzyme responsible for nitrite reduction in dissimilatory denitrification. Proton nuclear magnetic resonance spectroscopy will be used to determine structure about the catalytic site heme groups, including the characterization of electronic states. Resonance raman spectroscopy will be used to investigate the vibrational states of the catalytic sites. The exact chemical structure of the prosthetic groups of the enzyme will be determined by purification of these from the intact enzyme and a structural determination through nuclear magnetic resonance, vibrational, optical, and mass spectroscopy. The interactions of oxides of nitrogen with whole cells will also be examined. The target enzyme system is representative of the general class of electron transport enzymes and proteins. Its study will promote the understanding on a fundamental level of this class which is responsible for energy production and respiration in all cells.

拟议的项目将确定酶的结构元素

项目成果

Carbon monoxide oxygenase activity of cytochrome cd1.

细胞色素 cd1 的一氧化碳加氧酶活性。

• DOI: 10.1021/bi00414a064
• 发表时间: 1988
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Timkovich,R;Thrasher,JS
• 通讯作者: Thrasher,JS

NMR comparison of prokaryotic and eukaryotic cytochromes c.

原核和真核细胞色素的 NMR 比较 c．

• DOI: 10.1021/bi00473a012
• 发表时间: 1990
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Chau,MH;Cai,ML;Timkovich,R
• 通讯作者: Timkovich,R

Hierarchy of globin complexes. The quaternary structure of the extracellular chlorocruorin of Eudistylia vancouverii.

珠蛋白复合物的层次结构。

• DOI: 10.1016/0022-2836(91)90596-x
• 发表时间: 1991
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Qabar,AN;Stern,MS;Walz,DA;Chiu,JT;Timkovich,R;Wall,JS;Kapp,OH;Vinogradov,SN
• 通讯作者: Vinogradov,SN

Hydride transfer stereospecificity of rat liver aldehyde dehydrogenases.

大鼠肝醛脱氢酶的氢化物转移立体特异性。

• 发表时间: 1987
• 期刊: The Journal of biological chemistry
• 作者: Jones,KH;Lindahl,R;Baker,DC;Timkovich,R
• 通讯作者: Timkovich,R

1H NMR investigation of cytochrome cd1: complexes with electron-donor proteins.

细胞色素 cd1 的 1 H NMR 研究：与电子供体蛋白的复合物。

• DOI: 10.1021/bi00353a022
• 发表时间: 1986
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Timkovich,R