STRUCTURE OF CYTOCHROMES

细胞色素的结构

• 批准号: 3302338
• 负责人: RUSSELL TIMKOVICH
• 金额: $ 7.61万
• 依托单位: UNIVERSITY OF ALABAMA IN TUSCALOOSA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3302338
• 关键词: Pseudomonas; Pseudomonas aeruginosa; bacterial proteins; carbon; chemical bond resonance; chemical stability; conformation; cytochrome c; electron transport; electronic spectra; molecular dynamics; nuclear magnetic resonance spectroscopy; porphyrin structure; protein folding; protein purification; protein structure function; stable isotope

The overall objective is to determine how the structure and dynamics of low molecular weight cytochromes relate to their function as electron transport catalysts. Cytochromes are ubiquitous electron transport proteins found in all cell lines. An understanding of their function will provide fundamental knowledge into a central process of energy metabolism. The main technique will be NMR spectroscopy with accent on two dimensional solution studies. The primary experimental systems are cytochromes c551 from Pseudomonas aeruginosa and Pseudomonas stutzeri. The specific aims are to (a) assign their proton spectra in the oxidized and reduced forms, (b) compare their structures to each other and to larger eukaryotic cytochromes, (c) determine the kinetics of amide NH exchange as a probe for internal flexibility, and, (d) assess the importance of non-amino acid elements in determining the final tertiary conformation.

总的目标是确定低气压的结构和动力学 细胞色素分子量与其电子传递功能有关 催化剂的 细胞色素是普遍存在的电子传递蛋白， 所有细胞系。 了解它们的功能， 基础知识转化为能量代谢的中心过程。 的 主要技术将是核磁共振光谱学，重点是二维 解决方案研究 主要的实验系统是细胞色素c551 来自铜绿假单胞菌和施氏假单胞菌。 具体目标 是（a）分配它们的氧化和还原形式的质子光谱， (b)将它们的结构相互比较， 细胞色素，（c）确定酰胺NH交换的动力学作为探针 内部灵活性，和（d）评估非氨基酸的重要性 决定最终三级构象的元素。