STRUCTURE OF SULFGLOBINS

硫球蛋白的结构

• 批准号: 3293693
• 负责人: RUSSELL TIMKOVICH
• 金额: $ 2.82万
• 依托单位: UNIVERSITY OF ALABAMA IN TUSCALOOSA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3293693
• 关键词: chemical structure function; hemoglobin; myoglobin; nuclear magnetic resonance spectroscopy; selenium; sulfur compounds; thioether

Sulfhemoglobin and sulfmyoglobin are two unusual nonfunctional forms of the native globins found in blood and tissue. They may also be formed in vitro under controlled conditions. Past research has shown that the sulfglobins are produced by the covalent addition of some sulfur containing moiety to the heme prosthetic group of the native globins. This project will determine the structure of these unusual derivatives. The modified heme will be isolated and stabilized by choice of extraction conditions or by conversion to a more stable derivative that retains a record of the original modification. The isolated heme will be characterized by proton nuclear magnetic resonance, optical, infra-red spectroscopy, and mass spectrometry to determine its structure. In parallel experiments, the solution structure of the whole protein sulfmyoglovin will be determined by proton nuclear magnetic resonance.

硫血红蛋白和硫肌红蛋白是两种不常见的无功能蛋白