GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
批准号:
3286192
负责人:
Stanley R. Maloy
金额:
$11.38万
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-04-01 至 1993-03-31
关键词:
DNA Salmonella typhimurium bacterial genetics binding proteins electron transport enzyme mechanism flavoproteins genetic manipulation genetic mapping genetic recombination laboratory rabbit membrane proteins molecular cloning mutant nucleic acid sequence protein reconstitution protein structure regulatory gene
中文摘要
本研究的目的是确定黄素
沙门氏菌中的酶与膜相互作用
鼠伤寒。 这项工作将侧重于两个主要专题:
1. put操纵子的调控。 put操纵子有一个独特的
似乎是由putA基因介导的控制机制
产物,脯氨酸氧化酶。 当外源脯氨酸可用时,
脯氨酸氧化酶与细胞膜结合,
黄素脱氢酶;但在没有脯氨酸的情况下,
在细胞质中积累,
阻遏物 确定脯氨酸氧化酶如何控制其自身
表达可能有助于我们了解其他膜结合蛋白如何
调节蛋白起作用。 此外,
putA突变提供了一个膜的遗传选择,
互作突变体
2. 黄素氧化酶-膜相互作用。 黄素
酶必须与膜结合,
与电子传递链适当地相互作用。 但
还不知道它们是如何与细胞膜相互作用的,
与之相互作用的电子传递链的组分
直接. 脯氨酸氧化酶互作的遗传研究
提供了一个模型系统,
蛋白质是如何在体内与细胞膜相互作用的。 蛋白质-
膜所需的蛋白质和蛋白质-脂质相互作用
关联可以通过分离突变体来确定,
防止脯氨酸氧化酶与膜结合。 遗传
对这些突变体的生化分析将表明
膜组分直接与黄素相互作用
以及需要哪些类型的交互
黄素氧化酶和膜电子
运输链。 这些突变体应该提供洞察力,
一个普遍的生物学问题,
与膜相互作用。
英文摘要
The objective of this study is to determine how flavin
dehydrogenases interact with the membrane in Salmonella
typhimurium. This work will focus on two main topics:
1. Regulation of the put operon. The put operon has a unique
control mechanism that seems to be mediated by the putA gene
product, proline oxidase. When exogenous proline is available,
proline oxidase binds to the membrane where it functions as a
flavin dehydrogenase; but in the absence of proline, it
accumulates in the cytoplasm where it acts as an autogenous
repressor. Determining how proline oxidase controls its own
expression may help us understand how other membrane-bound
regulatory proteins work. In addition, the regulatory properties of
putA mutations provide a genetic selection for membrane
interaction mutants.
2. Flavin dehydrogenase-membrane interactions. Flavin
dehydrogenases must associate with the membrane in order to
interact properly with the electron transport chain. However, it
is not known how they interact with the membrane or what
components of the electron transport chain they interact with
directly. Genetic studies on the interaction of proline oxidase
with the membrane provides a model system for understanding
how proteins interact with membranes in vivo. The protein-
protein and protein-lipid interactions required for membrane
association can be determined by isolation of mutants that
prevent proline oxidase from binding to the membrane. Genetic
and biochemical analysis of these mutants will indicate what
membrane component(s) directly interact with flavin
dehydrogenases and what kinds of interactions are required
between flavin dehydrogenases and the membrane electron
transport chain. Such mutants should provide insight into the
general biological problem of how peripheral membrane proteins
interact with the membrane.
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会议论文
STRUCTURE AND FUNCTION OF PROLINE PERMEASE
-
批准号:3295959
-
项目类别:
-
资助金额:$8.4万
-
财政年份:1988
-
负责人:Stanley R. Maloy
-
依托单位:
STRUCTURE AND FUNCTION OF PROLINE PERMEASE
-
批准号:3295956
-
项目类别:
-
资助金额:$8.3万
-
财政年份:1988
-
负责人:Stanley R. Maloy
-
依托单位:
STRUCTURE AND FUNCTION OF PROLINE PERMEASE
-
批准号:3295958
-
项目类别:
-
资助金额:$8.16万
-
财政年份:1988
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:2177549
-
项目类别:
-
资助金额:$22.27万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286190
-
项目类别:
-
资助金额:$8.76万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286188
-
项目类别:
-
资助金额:$20.22万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:2684793
-
项目类别:
-
资助金额:$26.5万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:6179670
-
项目类别:
-
资助金额:$27.08万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:2177548
-
项目类别:
-
资助金额:$22.4万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286186
-
项目类别:
-
资助金额:$8.27万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:2022036
-
项目类别:
-
资助金额:$25.66万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:2177547
-
项目类别:
-
资助金额:$21.07万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286187
-
项目类别:
-
资助金额:$11.25万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286191
-
项目类别:
-
资助金额:$11.47万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286193
-
项目类别:
-
资助金额:$11.8万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286194
-
项目类别:
-
资助金额:$12.16万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:2900606
-
项目类别:
-
资助金额:$26.32万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
-
批准号:3286189
-
项目类别:
-
资助金额:$7.83万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
海外基金