STRUCTURE AND FUNCTION OF PROLINE PERMEASE
STRUCTURE AND FUNCTION OF PROLINE PERMEASE
批准号:
3295956
负责人:
Stanley R. Maloy
金额:
$8.3万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-02-01 至 1991-01-31
中文摘要
传输是入口中必不可少的且通常限制速率的步骤
英文摘要
Transport is an essential and often rate limiting step in the entry
of nutrients and ions into cells. However, despite many elegant
biophysical studies on transport proteins, very little is known
about the actual molecular mechanisms of transport. The ease of
isolation and molecular characterization of proline transport
mutants in Salmonella typhimurium makes this a good model
system for studying the molecular mechanism of sodium-driven
transport. We plan to isolate mutants of S. typhimurium
defective for proline transport in order to identify the active site
or proline permease. By selecting for mutants with altered
substrate of counter-ion specificity, domains of the permease
involved in substrate binding and translocation can be identified.
DNA sequence analysis of the mutants will identify the amino
acid residues that directly interact with the substrate and
counter-ion. The kinetics of substrate binding and translocation
will be measured for each mutant. The phenotype of mutants
with specific amino acid substitutions may indicate the function
of these amino acids in substrate translocation. Site directed
mutagenesis of the residues implicated in transport will allow us
to test the predicted role of specific amino acids in substrate
translocation. In order to determine the position of the active
site in the protein, the structure of proline permease will be
predicted from the DNA sequence of the putP gene and the
topology of the protein in the membrane will be determined using
gene fusions. Such genetic and biochemical analysis of proline
permease may elucidate the structure and function of ion-driven
active transport systems.
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STRUCTURE AND FUNCTION OF PROLINE PERMEASE
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批准号:3295959
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项目类别:
-
资助金额:$8.4万
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财政年份:1988
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负责人:Stanley R. Maloy
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依托单位:
STRUCTURE AND FUNCTION OF PROLINE PERMEASE
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批准号:3295958
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项目类别:
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资助金额:$8.16万
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财政年份:1988
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负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:2177549
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项目类别:
-
资助金额:$22.27万
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财政年份:1985
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负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286190
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项目类别:
-
资助金额:$8.76万
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财政年份:1985
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负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286188
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项目类别:
-
资助金额:$20.22万
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财政年份:1985
-
负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:2684793
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项目类别:
-
资助金额:$26.5万
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财政年份:1985
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负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:6179670
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项目类别:
-
资助金额:$27.08万
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财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:2177548
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项目类别:
-
资助金额:$22.4万
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财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286192
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项目类别:
-
资助金额:$11.38万
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财政年份:1985
-
负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286186
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项目类别:
-
资助金额:$8.27万
-
财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:2022036
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项目类别:
-
资助金额:$25.66万
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财政年份:1985
-
负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:2177547
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项目类别:
-
资助金额:$21.07万
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财政年份:1985
-
负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286187
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项目类别:
-
资助金额:$11.25万
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财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286191
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项目类别:
-
资助金额:$11.47万
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财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286194
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项目类别:
-
资助金额:$12.16万
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财政年份:1985
-
负责人:Stanley R. Maloy
-
依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286193
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项目类别:
-
资助金额:$11.8万
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财政年份:1985
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负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:2900606
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项目类别:
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资助金额:$26.32万
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财政年份:1985
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负责人:Stanley R. Maloy
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依托单位:
GENETICS OF FLAVIN DEHYDROGENASE-MEMBRANE INTERACTIONS
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批准号:3286189
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项目类别:
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资助金额:$7.83万
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财政年份:1985
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负责人:Stanley R. Maloy
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依托单位:
海外基金