MODIFIED NMR PULSE SHAPES FOR BIOMOLECULES AND IMAGING

用于生物分子和成像的改进核磁共振脉冲形状

• 批准号: 3287692
• 负责人: Warren S Warren
• 金额: $ 5.65万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3287692
• 关键词: mathematical model; nuclear magnetic resonance spectroscopy

Nuclear magnetic resonance (NMR) has developed into an extremely powerful technique for probing the structure of biologically interesting molecules and for imaging tissues with nondestructive radiation. Virtually all NMR experiments have used radiation pulses with rectangular envelopes, since the theory is particularly simple for this special case. We propose the application of radiation pulses with crafted, nonrectangular shapes. We have recently published a theoretical paper which shows that such shapes give better resolution (which is important for interpreting the complex spectra of proteins), less heating, and better reproducibility. We have designed particularly simple shapes to replace the pulses in conventional sequences. This proposal lets us test these shapes experimentally, and extends our work to generation of narrowband and narrow reject pulses, measurements of spatially resolved diffusion constants, correction of spectrometer imperfections, and reduction of noise in sophisticated two-dimensional experiments. This approach promises to significantly enhance the applicability of NMR to extremely complex systems.

核磁共振已经发展成为一种非常强大的 探测生物感兴趣的分子结构的技术 以及对非破坏性辐射的组织进行成像。几乎所有的核磁共振 实验使用了矩形包络的辐射脉冲，因为 对于这种特殊情况，这个理论特别简单。我们建议 应用精心制作的非矩形形状的辐射脉冲。我们 最近发表了一篇理论论文，表明这些形状 提供更好的分辨率(这对于解释复杂的 蛋白质光谱)，加热更少，重复性更好。我们有 设计了特别简单的形状来取代传统的脉冲 序列。这项提议让我们可以通过实验测试这些形状，并且 将我们的工作扩展到产生窄带和窄抑制脉冲， 空间分辨扩散常数的测量.校正 分光计的缺陷和尖端噪声的降低 二维实验。这种方法有望显著地 增强了核磁共振对极其复杂体系的适用性。