BRAIN MONOAMINES AND LUTEINIZING HORMONE SECRETION

脑单胺和黄体生成素的分泌

• 批准号: 3312270
• 负责人: WILLIAM R CROWLEY
• 金额: $ 6.74万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-12-01 至 1987-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3312270
• 关键词: 6 hydroxydopamine; brain metabolism; denervation; dopamine; epinephrine; estradiol; high performance liquid chromatography; hormone regulation /control mechanism; luteinizing hormone; neurochemistry; neuroendocrine system; neurotoxins; neurotransmitter metabolism; norepinephrine; optics; pituitary gonadal axis; progesterone; prolactin; radioimmunoassay; radiotracer; serotonin; sympathetic nervous system; thin layer chromatography

The overall objective of the proposed research is to investigate the neurochemical mechanisms by which estradiol and progesterone control the secretion of luteinizing hormone (LH) and prolactin (PRL) from the anterior pituitary gland. This research will test the hypothesis that ovarian hormones regulate LH and PRL secretion by activating endogenous opiate peptide systems that exert localized control over the release of norepinephrine, epinephrine and serotonin. It is also hypothesized that monoaminergic control over LH and PRL is modified by the presence or absence of testosterone during a perinatal critical period. These hypotheses will be tested by the following approaches. First, ovarian hormones will be administered in specific regimens that depress or elevate LH and PRL, and the effects of these treatments on the turnover of serotonin in discrete brain nuclei will be assessed. This will identify those sites where ovarian hormones alter serotonergic activity. The second phase will attempt to establish causal relationships between the changes of serotonin activity and the changes in LH and PRL induced by the ovarian hormones. This will involve attempts to alter the expected ovarian hormonal feedback effects by producing local denervations with the neurotoxic indoleamine, 5,7-dihydroxytryptamine. The third phase will test whether ovarian hormones interact with endogenous opiate neuropeptides that influence the release of monoamines to regulate LH and PRL secretion. This will be accomplished by testing whether ovarian hormonal effects on both pituitary hormone secretion and on monoamine turnover can be affected by opiate agonists and antagonists. A fourth aspect of the project will investigate whether castration of neonatal males or administration of testosterone to neonatal females, manipulations that alter ovarian hormonal control over LH and PRL in adulthood, also alter the effects of ovarian hormones on LH-releasing hormone and on monoamine turnover.

拟议研究的总体目标是调查 神经化学机制，雌二醇和孕酮控制 促黄体生成素（LH）和催乳素（PRL）从前部分泌 脑下垂体 这项研究将验证卵巢癌的假设， 激素通过激活内源性阿片调节LH和PRL分泌 肽系统对释放施加局部控制， 去甲肾上腺素肾上腺素和血清素 也有人假设， 对LH和PRL的单胺能控制通过存在或 在围产期的关键时期缺乏睾丸激素。 这些 将通过以下方法检验假设。 一、卵巢 激素将以特定的方案施用， LH和PRL，以及这些治疗对 将评估离散脑核中的5-羟色胺。 这将确认 卵巢激素改变肾上腺素能活性的部位。 第二 阶段将试图建立变化之间的因果关系， 血清素活性及卵巢刺激引起的LH和PRL的变化 荷尔蒙 这将涉及试图改变预期的卵巢 激素反馈的影响，通过产生局部去神经与 神经毒性吲哚胺，5，7-二羟色胺。 第三阶段将进行测试 卵巢激素是否与内源性阿片神经肽相互作用， 影响单胺的释放，调节LH和PRL的分泌。 这 将通过测试卵巢激素是否对两者都有影响来完成 垂体激素分泌和对单胺周转的影响， 阿片激动剂和拮抗剂。 该项目的第四个方面将 研究是否对新生雄性进行去势或给予 睾丸激素对新生儿女性，改变卵巢激素的操作， 控制LH和PRL在成年期，也改变卵巢的影响， 激素对LH释放激素和单胺周转的影响。