Sequencing and assembling microbial community metagenomes in real-time

实时测序和组装微生物群落宏基因组

• 批准号: DP170102626
• 负责人: Prof Lachlan Coin
• 金额: $ 29.36万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170102626
• 关键词: Sequencing; assembling; microbial; community; metagenomes

This project aims to assemble metagenomes directly from environmental samples using nanopore sequencing. Short-read approaches to metagenomics cannot assemble mixed genomes from an environmental sample, so focus on describing which species and genes are present. Long-read nanopore sequencing enables the assembly of full genomes of multiple species in a sample. Assembling complete genomes in important resources such as water and soil should lead to deeper understanding of the dynamics, variation and transfer of genetic material within these resources’ microbial communities, strategies to manage microbial diversity, and improved productivity and long-term sustainability for these resources.

该项目旨在使用纳米孔测序直接从环境样本中组装宏基因组。宏基因组学的短读方法无法从环境样本中组装混合基因组，因此重点描述存在哪些物种和基因。长读长纳米孔测序能够组装样本中多个物种的完整基因组。在水和土壤等重要资源中组装完整的基因组应该有助于更深入地了解这些资源的微生物群落内遗传物质的动态、变异和转移，制定管理微生物多样性的策略，并提高这些资源的生产力和长期可持续性。