REGULATION OF CECROPIN AND ATTACIN GENE EXPRESSION

天蚕素和 Attacin 基因表达的调控

• 批准号: 3300131
• 负责人: PETER DUNN
• 金额: $ 10.74万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3300131
• 关键词: bacterial proteins; cellular immunity; fat body; gene expression; genetic regulation; genetic transcription; hemolymph; insect control; molecular genetics; nucleic acid hybridization; peptidoglycan; protein biosynthesis; radionuclides; structural genes

The molecular analysis of gene expression in insects, with the notable exception of Drosophila melanogaster, lags behind those in prokaryotes, yeast, and vertebrates. A well characterized insect system amenable to dissection at the molecular level is provided by the antibacterial response of lepidopteran insects. Resistance to infection in insects is accomplished largely via structural barriers that limit access to the hemocoel. If these structural barriers are disrupted via injury or attack, potential pathogens or parasites may gain access to the hemocoel. To survive these infections, insects have evolved a set of active secondary defenses that (i) limit hemolymph loss and heal wounds, (ii) detect and eliminate invaders, and (iii) protect the injured host while structural barriers and depleted defenses are restored. One component of the active defensive arsenal against bacteria is the regulated synthesis of antibacterial, hemolymph proteins. The long term goal of the proposed research is to discover molecular mechanisms regulating the expression of genes encoding insect antibacterial proteins. The present proposal focuses on transcriptional regulation of antibacterial protein genes, gene structure, and tissue specific expression. Specific objectives are: (1) to determine the contribution of transcriptional control to peptidoglycan regulation of fat body cecropin-like protein (CLP) and attacin-like protein (ALP) synthesis; (2) to isolate and characterize M. sexta genes encoding CLP and ALP; (3) to analyze secondary structural elements associated with CLP and ALP genes in naive and peptidoglycan-induced fat body; and (4) to determine whether established lepidopteran cell lines exhibit enhanced transcription from antibacterial protein genes and synthesize elevated levels of CLP and ALP in response to peptidoglycan elicitors. The proposed research will yield new information concerning (i) the insect host-pathogen/parasite interaction and (ii) the structure and regulation of insect genes. This knowledge may contribute to the management of insect vectors of human and animal disease.

昆虫基因表达的分子分析 黑腹果蝇的显着例外，落后于 prokaryotes, yeast, and vertebrates. A well characterized insect 提供了适合分子水平解剖的系统 通过鳞翅目昆虫的抗菌反应。 昆虫对感染的抵抗力主要是通过 限制进入血腔的结构障碍。 如果这些 结构障碍因伤害或攻击而被破坏，潜在的 病原体或寄生虫可能会进入血腔。 为了生存 这些感染，昆虫进化出了一套活跃的次级 (i) 限制血淋巴流失和愈合伤口，(ii) 检测的防御 并消灭入侵者，以及（iii）保护受伤的宿主，同时 结构性障碍和耗尽的防御得到恢复。 一 对抗细菌的主动防御武器库的组成部分是 调节抗菌、血淋巴蛋白的合成。 拟议研究的长期目标是发现 调节编码基因表达的分子机制 insect antibacterial proteins. 本提案的重点是 抗菌蛋白基因的转录调控 structure, and tissue specific expression. 具体目标 是：（1）确定转录控制的贡献 肽聚糖对脂肪体天蚕素样蛋白 (CLP) 的调节 and attacin-like protein (ALP) synthesis; (2) 隔离和 表征编码 CLP 和 ALP 的 M. sexta 基因； (3) 分析 与 CLP 和 ALP 基因相关的二级结构元件 幼稚和肽聚糖诱导的脂肪体； (4) 确定 建立的鳞翅目细胞系是否表现出增强 抗菌蛋白基因转录并合成 肽聚糖引起的 CLP 和 ALP 水平升高 诱导子。 拟议的研究将产生有关以下方面的新信息：（i） 昆虫宿主-病原体/寄生虫相互作用和（ii）结构 and regulation of insect genes. This knowledge may contribute to 人类和动物疾病昆虫媒介的管理。