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REGULATION OF CECROPIN AND ATTACIN GENE EXPRESSION

天蚕素和 Attacin 基因表达的调控

基本信息

批准号：
3300132
负责人：
PETER DUNN
金额：
$ 11.24万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-04-01 至 1992-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3300132
关键词：
Lepidoptera bacterial proteins cellular immunity fat body gene expression genetic regulation genetic transcription hemolymph insect control molecular genetics nucleic acid hybridization peptidoglycan protein biosynthesis radionuclides structural genes

项目摘要

The molecular analysis of gene expression in insects, with the notable exception of Drosophila melanogaster, lags behind those in prokaryotes, yeast, and vertebrates. A well characterized insect system amenable to dissection at the molecular level is provided by the antibacterial response of lepidopteran insects. Resistance to infection in insects is accomplished largely via structural barriers that limit access to the hemocoel. If these structural barriers are disrupted via injury or attack, potential pathogens or parasites may gain access to the hemocoel. To survive these infections, insects have evolved a set of active secondary defenses that (i) limit hemolymph loss and heal wounds, (ii) detect and eliminate invaders, and (iii) protect the injured host while structural barriers and depleted defenses are restored. One component of the active defensive arsenal against bacteria is the regulated synthesis of antibacterial, hemolymph proteins. The long term goal of the proposed research is to discover molecular mechanisms regulating the expression of genes encoding insect antibacterial proteins. The present proposal focuses on transcriptional regulation of antibacterial protein genes, gene structure, and tissue specific expression. Specific objectives are: (1) to determine the contribution of transcriptional control to peptidoglycan regulation of fat body cecropin-like protein (CLP) and attacin-like protein (ALP) synthesis; (2) to isolate and characterize M. sexta genes encoding CLP and ALP; (3) to analyze secondary structural elements associated with CLP and ALP genes in naive and peptidoglycan-induced fat body; and (4) to determine whether established lepidopteran cell lines exhibit enhanced transcription from antibacterial protein genes and synthesize elevated levels of CLP and ALP in response to peptidoglycan elicitors. The proposed research will yield new information concerning (i) the insect host-pathogen/parasite interaction and (ii) the structure and regulation of insect genes. This knowledge may contribute to the management of insect vectors of human and animal disease.

昆虫基因表达的分子分析，果蝇是一个显著的例外，原核生物、酵母和脊椎动物。一种特征鲜明的昆虫提供了一种适于在分子水平上解剖的系统鳞翅目昆虫的抗菌反应。昆虫对感染的抗性主要通过以下途径实现：限制进入血腔的结构屏障。如果这些结构性屏障因受伤或攻击而被破坏，病原体或寄生虫可以进入血腔。生存这些感染，昆虫已经进化出一套活跃的次级防御：（i）限制血淋巴损失并治愈伤口，（ii）检测和消除入侵者，和（iii）保护受伤的主机，结构性屏障和耗尽的防御得以恢复。一对细菌的主动防御武器的组成部分是调节抗菌血淋巴蛋白的合成。这项研究的长期目标是发现调控基因表达的分子机制昆虫抗菌蛋白本建议的重点是抗菌蛋白基因的转录调控结构和组织特异性表达。具体目标主要有：（1）确定转录调控的贡献肽聚糖调节脂肪体天蚕素样蛋白（CLP）和粘附素样蛋白（ALP）的合成;（2）分离和描述M.编码CLP和ALP的sexta基因;（3）分析与CLP和ALP基因相关的二级结构元件天然和肽聚糖诱导的脂肪体;和（4）确定已建立的鳞翅目细胞系是否表现出增强的抗菌蛋白基因的转录和合成肽聚糖引起的CLP和ALP水平升高诱导剂。拟议的研究将产生新的信息，涉及（i）昆虫宿主-病原体/寄生虫相互作用和（ii）结构和昆虫基因的调控。这些知识可能有助于人类和动物疾病的昆虫媒介的管理。