ROLE OF RECEPTORS IN ESTROGEN ACTION IN THE UTERUS

这项研究的长期目标是了解 雌激素受体在调节子宫生长和功能中的作用 阐明了重要的受体介导的事件， 雌激素对靶细胞基因功能的调节。 这要作 通过研究类固醇激素调节的 与雌激素依赖性受体相关的标志蛋白过氧化物酶 调节和染色质结合，并通过这种手段来发现 雌激素作用在分子水平上的生物学重要细节。 攻击计划是在老鼠身上使用几个实验系统， 小鼠和仓鼠以及各种生物学上有意义的激素治疗 发现过氧化物酶的激素控制的性质的协议 表达，并将这些标志物的变化与雌激素 受体相互作用及其活化、核结合和染色质 定位，最终是基因调控。 鼠子宫抗体 过氧化物酶，我们将着手研究定量激素调节 过氧化物酶mRNA，免疫化学可检测的过氧化物酶多肽和 酶，以澄清转录，加工， 翻译和翻译后的控制作用的激素在 子宫 用我们实验室新开发的试剂， 标记过氧化物酶的抗体和雌激素受体的抗体， 我们有独特的试剂和能力进行研究， 阐明雌激素受体介导的调节的细节 哺乳动物生殖道中基因表达的变化。 这些研究可以 有望提高我们对子宫内分泌学的认识 在生物化学层面上。

The long term objective of this research is to understand the role of estrogen receptors in the regulation of uterine growth and function by elucidating the important receptors mediated events which are required for estrogen regulation of gene function in target cells. This is to be accomplished by the study of the steroid hormone regulated expression of the marker protein, peroxidase, correlated with estrogen-dependent receptor modulation and chromatin binding and by this means to discover the biologically important details of estrogen action at the molecular level. The plan of attack is to use several experimental systems in the rat, mouse, and hamster and various biologically meaningful hormone treatment protocols to discover the nature of hormonal controls of peroxidase expression, and to relate these changes in marker with the estrogen receptor interaction and its activation, nuclear binding, and chromatin localization, and eventually gene regulation. With antibody to rat uterine peroxidase we will embark on studies to quantitate the hormonal regulation of peroxidase mRNA, immunochemically detectable peroxidase polypeptide and enzyme in order to clarify the nature of the transcriptional, processing, translational and post-translational controls effected by hormones in the uterus. With the newly developed reagents in our laboratory, specifically antibody to the marker peroxidase and antibodies to the estrogen receptor, we have the unique reagents and capability to carry out studies which can clarify the intimate details of the estrogen receptor-mediated regulation of gene expression in the mammalian reproductive tract. These studies can be expected to improve our understanding of the endocrinology of the uterus at the biochemical level.