PHYSIOLOGY OF PITUITARY FOLLICULOSTELLATE CELLS

垂体滤泡细胞的生理学

• 批准号: 3320818
• 负责人: JAMES JEFFREY MULCHAHEY
• 金额: $ 6.91万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3320818
• 关键词: cyclic AMP; electron microscopy; follicle stimulating hormone; hormone regulation /control mechanism; laboratory rat; luteinizing hormone; physiology; pituitary gonadal axis; prolactin; radiotracer; somatotropin; tissue /cell culture

The long range goal is to more fully understand the physiology of the pituitary folliculostellate (FS) cell. No unequivocal function has been demonstrated for these cells. The hypothesis to be tested is that FS cells affect anterior pituitary (AP) physiology by altering the local extra- or intracellular levels of 3', 5' cyclic adenosine monophosphate (cAMP) and Ca2+. Altered local levels of cAMP and Ca2+ then affect the rate of hormone secretion from secretory AP cells. The specific aims are to: 1) develop and validate an in vitro cell culture paradigm for rat FS cells, 2) describe the morphology and ultrastructure of FS cells in these cultures and analyze the patterns and nature of FS cell association with AP cells, 3) determine the effects of FS cells on hormone secretion from other AP cells and 4) elucidate the mechanisms by which FS cells might communicate with other FS cells and influence hormone secretion from AP cells. These aims will be achieved by developing monolayer cultures of FS cells on an extracellular matrix. Highly enriched populations of FS cells will be obtained by separation of monodispersed pituitary cells by unit gravity sedimentation. These cells will be used then to initiate the monolayer FS cell cultures. The monolayer cultures will be used for studies of cAMP production in basal and hormone-stimulated states. Intracellular Ca2+ mobilization will also be examined in these states with Quin 2 fluorescence. Cell-cell connections will be examined using a dye coupling model. Finally AP hormone (growth hormone, luteinizing hormone and prolactin) secretion from individual AP cells, either associated with FS cells or unassociated, will be examined with reverse hemolytic plaque assays for these hormones. The effects of FS cells on hormone secretion, secretagogue responsiveness and the spectrum of secretagogues to which AP cells respond will be investigated. These studies of FS cell physiology are likely to provide additional insight into the growth of the AP in fetal development and various neoplastic conditions.

长期目标是更全面地了解人体的生理学 垂体滤泡细胞（FS）。 没有明确的功能 证明了这些细胞。 要检验的假设是 FS 细胞 通过改变局部的外部或外部影响垂体前叶（AP）的生理机能 细胞内 3'、5' 环磷酸腺苷 (cAMP) 和 钙2+。 改变局部 cAMP 和 Ca2+ 水平会影响 分泌性AP细胞分泌激素。 具体目标是：1） 开发并验证大鼠 FS 细胞的体外细胞培养范例，2) 描述这些培养物中 FS 细胞的形态和超微结构 并分析 FS 细胞与 AP 细胞关联的模式和性质， 3)确定FS细胞对其他AP激素分泌的影响 细胞和 4) 阐明 FS 细胞可能通讯的机制 与其他 FS 细胞相互作用并影响 AP 细胞的激素分泌。 这些 目标将通过在 FS 细胞上开发单层培养物来实现 细胞外基质。 高度富集的 FS 细胞群将 通过单位重力分离单分散垂体细胞获得 沉降。 这些细胞将用于启动单层 FS 细胞培养物。 单层培养物将用于 cAMP 的研究 基础状态和激素刺激状态下的生产。 细胞内Ca2+ Quin 2 也将在这些州检查动员情况 荧光。 将使用染料耦合检查细胞与细胞的连接 模型。 最后是AP激素（生长激素、黄体生成激素和 催乳素）从单个 AP 细胞分泌，或者与 FS 相关 细胞或无关的，将用反向溶血斑块进行检查 对这些激素进行测定。 FS细胞对激素分泌的影响， AP 的促分泌素反应性和促分泌素谱 将研究细胞的反应。 FS细胞生理学的这些研究 可能会为胎儿 AP 的生长提供更多见解 发育和各种肿瘤状况。