PHYSIOLOGY OF PITUITARY FOLLICULOSTELLATE CELLS

长期的目标是更全面地了解 垂体滤泡星状细胞(FS)。没有任何明确的功能是 为这些细胞演示了。需要检验的假设是FS细胞 改变局部外叶对垂体前叶(AP)生理的影响 细胞内3‘，5’环磷酸腺苷(CAMP)和 Ca2+。局部cAMP和Ca~(2+)水平的改变会影响 分泌型AP细胞的激素分泌。具体目标是：1) 建立和验证大鼠FS细胞的体外细胞培养模式，2) 描述在这些培养中的FS细胞的形态和超微结构 并分析FS细胞与AP细胞结合的模式和性质， 3)确定FS细胞对其他AP分泌激素的影响 细胞和4)阐明FS细胞可能的通讯机制 并影响AP细胞的激素分泌。这些 目标将通过开发单层培养的FS细胞在 细胞外基质。高度浓缩的FS细胞群体将是 通过单位重力分离单一分散的脑垂体细胞而获得 沉积作用。然后这些电池将被用来启动单层FS 细胞培养。单层培养将用于cAMP的研究 在基础状态和激素刺激状态下的生产。细胞内钙离子 在这些州也将使用Quin 2进行动员检查 荧光。细胞与细胞的连接将使用染料偶联进行检查 模特。最后是AP激素(生长激素、黄体生成素和 单个AP细胞分泌催乳素)，或与FS相关 细胞或无关联，将用反向溶血斑块进行检查 这些荷尔蒙的化验。FS细胞对激素分泌的影响 促分泌剂的反应性和AP对促分泌剂的谱 将对细胞反应进行调查。FS细胞生理学的这些研究 可能为胎儿AP的生长提供更多的洞察力 发展和各种肿瘤条件。

The long range goal is to more fully understand the physiology of the pituitary folliculostellate (FS) cell. No unequivocal function has been demonstrated for these cells. The hypothesis to be tested is that FS cells affect anterior pituitary (AP) physiology by altering the local extra- or intracellular levels of 3', 5' cyclic adenosine monophosphate (cAMP) and Ca2+. Altered local levels of cAMP and Ca2+ then affect the rate of hormone secretion from secretory AP cells. The specific aims are to: 1) develop and validate an in vitro cell culture paradigm for rat FS cells, 2) describe the morphology and ultrastructure of FS cells in these cultures and analyze the patterns and nature of FS cell association with AP cells, 3) determine the effects of FS cells on hormone secretion from other AP cells and 4) elucidate the mechanisms by which FS cells might communicate with other FS cells and influence hormone secretion from AP cells. These aims will be achieved by developing monolayer cultures of FS cells on an extracellular matrix. Highly enriched populations of FS cells will be obtained by separation of monodispersed pituitary cells by unit gravity sedimentation. These cells will be used then to initiate the monolayer FS cell cultures. The monolayer cultures will be used for studies of cAMP production in basal and hormone-stimulated states. Intracellular Ca2+ mobilization will also be examined in these states with Quin 2 fluorescence. Cell-cell connections will be examined using a dye coupling model. Finally AP hormone (growth hormone, luteinizing hormone and prolactin) secretion from individual AP cells, either associated with FS cells or unassociated, will be examined with reverse hemolytic plaque assays for these hormones. The effects of FS cells on hormone secretion, secretagogue responsiveness and the spectrum of secretagogues to which AP cells respond will be investigated. These studies of FS cell physiology are likely to provide additional insight into the growth of the AP in fetal development and various neoplastic conditions.