TISSUE INTERACTIONS AND SEMINAL VESICLE SECRETION

组织相互作用和精囊分泌

• 批准号: 3321056
• 负责人: GERALD R CUNHA
• 金额: $ 10.69万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3321056
• 关键词: androgens; autoradiography; cell cell interaction; cell differentiation; cell growth regulation; epithelium; gel electrophoresis; gene expression; genetic manipulation; hormone receptor; hormone regulation /control mechanism; immunofluorescence technique; implant; laboratory mouse; laboratory rat; mesenchyme; nucleic acid hybridization; protein biosynthesis; radiotracer; secretion; seminal vesicles; testicular feminization; tissue /cell culture; tritium

The factors responsible for the development of an immune response to spermatozoa in some individuals but not in others, have not been eludicated. Recent data have led to the hypothesis, to be tested in the present application, that suppressor T lymphocytes present in semen and/or the ability of seminal fluid to activate suppressor cells aid in minimizing an immune response to spermatozoa in males and females. Sera and peripheral blood mononuclear cells (PBMN) will be obtained from 100 male and female partners with primary infertility and 35 male and female partners of fertile marriages. Semen will be obtained from all men. All sera and semen will be tested for sperm and seminal fluid-reactive antibodies. The numbers of suppressor and helper T lymphocytes in the ejaculates will be quantitated with monoclonal antibodies. The ability of seminal lymphocytes to block in vitro proliferation and immunoglobulin production by the semen donor's PBMN, by those of his spouse or of control PBMN, will be determined. Similarly, the induction of suppressor cell activity in PBMN of husband and wife following incubation with husband or control seminal fluid will be assessed. To ascertain the origin of the seminal fluid component which induces suppressor cell activity, pre- and post-vasovasostomy seminal fluids from 30 men, as well as epididymal fluid obtained at the time of surgery, will be incubated with PBMN and tested for their effect on lymphocyte proliferation and immunoglobulin production. To determine the cell type(s) required for seminal fluid activation of suppressor cells, macrophages, T lymphocytes and B lymphocytes will be isolated, incubated alone or in various combinations with seminal fluid and tested for immunosuppressive properties.

引起免疫反应的因素

项目成果

Morphogenetic and proliferative effects of testosterone and insulin on the neonatal mouse seminal vesicle in vitro.

睾酮和胰岛素对体外新生小鼠精囊的形态发生和增殖作用。

• DOI: 10.1210/endo-129-5-2289
• 发表时间: 1991
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Tsuji,M;Shima,H;Cunha,GR
• 通讯作者: Cunha,GR

Neonatal seminal vesicle mesenchyme induces a new morphological and functional phenotype in the epithelia of adult ureter and ductus deferens.

新生儿精囊间充质在成人输尿管和输精管上皮细胞中诱导新的形态和功能表型。

• DOI: 10.1242/dev.111.1.145
• 发表时间: 1991
• 期刊: Development (Cambridge, England)
• 作者: Cunha,GR;Young,P;Higgins,SJ;Cooke,PS
• 通讯作者: Cooke,PS

Effect of human recombinant mullerian inhibiting substance on isolated epithelial and mesenchymal cells during mullerian duct regression in the rat.

人重组苗勒管抑制物质对大鼠苗勒管退化过程中分离的上皮和间质细胞的影响。

• DOI: 10.1210/endo.131.3.1505479
• 发表时间: 1992
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Tsuji,M;Shima,H;Yonemura,CY;Brody,J;Donahoe,PK;Cunha,GR
• 通讯作者: Cunha,GR

In vitro androgen-induced growth and morphogenesis of the Wolffian duct within urogenital ridge.

体外雄激素诱导的泌尿生殖嵴内沃尔夫管的生长和形态发生。

• DOI: 10.1210/endo-128-4-1805
• 发表时间: 1991
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Tsuji,M;Shima,H;Cunha,GR
• 通讯作者: Cunha,GR

Mesenchymal-epithelial interactions in the growth and development of the prostate.

前列腺生长和发育中的间充质-上皮相互作用。

• DOI: 10.1007/978-1-4613-1595-7_9
• 发表时间: 1989
• 期刊: Cancer treatment and research
• 作者: Cunha,GR;Donjacour,AA
• 通讯作者: Donjacour,AA