RENAL TRANSPORT CARRIER FOR ORGANIC COMPOUNDS

有机化合物的肾脏运输载体

• 批准号: 3334145
• 负责人: CHARLES R. ROSS
• 金额: $ 14.35万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3334145
• 关键词: aminoacid transport; autoradiography; basolateral membrane; brush border membrane; chemical models; cholinesterases; chromatography; glucose transport; ion transport; kidney function; organic chemicals; p aminohippurate; phosphatidylcholines; phospholipids; quaternary ammonium compound; radiotracer; renal cortex; renal tubular transport; secretion; sphingomyelins

The objectives of the proposed research are to provide a detailed description of the mechanisms by which organic ions are secreted by the kidney. Since secretion is a transepithelial phenomenon, the approach selected is to study membrane transport in plasma membrane vesicles isolated from both faces of the renal cell. Our previous work demonstrated that "specifically-mediated" transport of both organic anions and organic cations occurred in both the basolateral and brush border membranes, and gave information on the relative contributions of the two membranes to the overall process. The present studies are designed to describe the underlying molecular mechanisms and to explain the energetics. Supposedly, they are "secondarily-active" transport mechanisms. Another objective is to compare the transporters located in either membrane on the basis of mechanism, chemical properties, and immunological cross reactivity. A third objective is to examine some amphoteric drugs to determine if they behave as anions or cations. A fourth objective is to isolate the transporters from both membranes in order to relate structure and function. The long-range goal is to understand the molecular mechanisms involved in secretion and to understand how secretion is regulated and controlled by the renal cell.

拟议研究的目标是提供一个详细的 对有机离子的分泌机制的描述 肾脏。由于分泌物是一种跨上皮现象，因此 被选中的是研究质膜囊泡中的膜运输 从肾细胞的两侧分离出来的。我们之前的工作证明了 有机阴离子和有机物质的“特定媒介”传输 阳离子既存在于基侧膜中，也存在于刷状边界膜中； 提供了关于这两种膜对 整体流程。目前的研究旨在描述 潜在的分子机制和解释能量学。据推测， 它们是“二次活跃”的运输机制。另一个目标是 比较位于这两种膜上的转运蛋白 作用机制、化学性质和免疫交叉反应。一个 第三个目标是检查一些两性药物，以确定它们是否 表现为阴离子或阳离子。第四个目标是隔离 两种膜上的转运蛋白，以便将结构和 功能。长期目标是了解分子机制。 参与分泌，并了解分泌是如何调节和 由肾细胞控制。