Quantifying reactive species in biological systems

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This project aims to discover how reactive oxygen and nitrogen species affect cell function, oxidative stress and cell dysfunction. The underlying mechanisms are often unknown because methods to measure reactive species and associated oxidative stress are inadequate. This project will synthesise multi-purpose probes and combine these with mass spectrometry-based analytics and mathematical modelling. This will allow concurrent quantification of reactive species in specific cellular compartments in a time-dependent manner that reflects their intricate interaction and metabolism in complex biological systems. This project could clarify whether and how reactive species affect cell function, ageing and disease.