MOLECULAR BIOLOGY OF THE MOUSE OOCYTE

小鼠卵母细胞的分子生物学

• 批准号: 3327174
• 负责人: SIDNEY STRICKLAND
• 金额: $ 13.89万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3327174
• 关键词: Schizosaccharomyces pombe; Xenopus; complementary DNA; egg /ovum; fertilization; gene expression; genetic library; genetic manipulation; genetic regulation; genetic translation; laboratory mouse; meiosis; messenger RNA; molecular cloning; nucleic acid hybridization; nucleic acid sequence; plasminogen activator; protein biosynthesis; translation factor

The mRNA for tissue plasminogen activator (t-PA) is present in primary mouse ooctyes but not translated. Upon meiotic maturation, this mRNA has 400-500 (A) residues added to the 3' end, begins to be translated, and is degraded. Using this system, and approaches developed to study t-PA mRNA recruitment, we propose the following: 1. To investigate using injection of RNA fragments and chimeric mRNAs the sequences in t-PA mRNA that determine its precise elongation, translational activation and subsequent degradation during oocyte maturation. 2. To search for molecules involved in oocyte maturation, meiosis and fertilization using cDNA libraries prepared from primary oocytes. 3. To assess whether information about meiosis gathered from other species, notably the fission yeast Schizosaccharomyces pombe and Xenopus laevis, can be used to further understand this process in mammals.

组织型纤溶酶原激活物（t-PA）的mRNA存在于原发性 mouse ooctyes但不翻译。在减数分裂成熟后，这种mRNA具有 400-500（A）残基添加到3'末端，开始被翻译，并且 退化了使用这个系统，以及研究t-PA mRNA的方法 在招聘方面，我们提出以下建议： 1.为了研究使用RNA片段和嵌合mRNA的注射， t-PA mRNA中决定其精确延伸、翻译 卵母细胞成熟过程中的激活和随后的降解。 2.寻找参与卵母细胞成熟、减数分裂和成熟的分子 使用从原代卵母细胞制备的cDNA文库进行受精。 3.为了评估是否从其他物种收集了有关减数分裂的信息， 特别是裂殖酵母裂殖酵母和非洲爪蟾， 用于进一步了解哺乳动物的这一过程。