MOLECULAR BIOLOGY OF THE MOUSE OOCYTE

小鼠卵母细胞的分子生物学

• 批准号: 3327170
• 负责人: SIDNEY STRICKLAND
• 金额: $ 11.24万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3327170
• 关键词: Schizosaccharomyces pombe; Xenopus; complementary DNA; egg /ovum; fertilization; gene expression; genetic library; genetic manipulation; genetic regulation; genetic translation; laboratory mouse; meiosis; messenger RNA; molecular cloning; nucleic acid hybridization; nucleic acid sequence; plasminogen activator; protein biosynthesis; translation factor

The mRNA for tissue plasminogen activator (t-PA) is present in primary mouse ooctyes but not translated. Upon meiotic maturation, this mRNA has 400-500 (A) residues added to the 3' end, begins to be translated, and is degraded. Using this system, and approaches developed to study t-PA mRNA recruitment, we propose the following: 1. To investigate using injection of RNA fragments and chimeric mRNAs the sequences in t-PA mRNA that determine its precise elongation, translational activation and subsequent degradation during oocyte maturation. 2. To search for molecules involved in oocyte maturation, meiosis and fertilization using cDNA libraries prepared from primary oocytes. 3. To assess whether information about meiosis gathered from other species, notably the fission yeast Schizosaccharomyces pombe and Xenopus laevis, can be used to further understand this process in mammals.

组织型纤溶酶原激活物（t-PA） mRNA在原发蛋白中存在