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CONSTRUCTION OF A FRAMEWORK MAP OF CHROMOSOME 18

CONSTRUCTION OF A FRAMEWORK MAP OF CHROMOSOME 18
18 号染色体框架图的构建
批准号:
3333647
负责人:
Thomas Conrad GILLIAM
金额:
$23.52万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-04-02 至 1994-03-31

项目摘要

项目成果

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中文摘要
翻译
提出了一种开发染色体遗传连锁图谱的策略
英文摘要
A strategy is presented to develop a genetic linkage map of chromosome 18. The map will consist of highly polymorphic index markers (heterozygosity > 0.7) spaced not more than 10-15 cM apart and spanning the full estimated genetic length of the chromosome. The linkage map will be composed of new markers developed in our laboratory, including both index and conventional (het < 0.7) markers, and other markers reported by the scientific community. we estimate that an additional 35 index markers are required to complete the project. A strategy is presented to isolate and characterize these markers and to determine their map location and order relative to existing chromosome 18 markers. All new markers will be characterized in the STS format. Through collaboration, new and existing markers will be physically mapped using radiation-reduced cell hybrids, somatic mapping panels, pulse-field gel electrophoresis mapping, and assignment to YAC contigs. Strategies to enrich for index markers indiscriminately across the chromosome as well as strategies to identify markers at discrete loci or chromosomal sub- regions are described. As a priority secondary to development of index markers, we will isolate, characterize, and genetically map less informative markers and begin the construction of a high resolution linkage map. Criteria will be developed to optimize the ultimate utility of the framework map. The optimal relationship between marker heterozygosity and interval spacing will be explored and we will assess the criteria for completion of the genetic map. we will continue to investigate the effects of commonly occurring errors on the genetic linkage map, and corrections for these errors will be incorporated into analysis of the chromosome 18 linkage data. Chromosome 18 resources and information generated as a part of the proposed research will be made immediately available to the research community.
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GENOMICS
  • 批准号:
    7714262
  • 项目类别:
  • 资助金额:
    $27.23万
  • 财政年份:
    2008
  • 负责人:
    Thomas Conrad GILLIAM
  • 依托单位:
CORE--GENOMICS /BIOINFORMATICS--ALZHEIMER'S AND AUTISM
Molecular Genetic Study of Fear and Anxiety
Gene-Enviroment Interaction in Cognition
国内基金
海外基金
小麦部分同源染色体(homoeologous chromosomes)间的定向重组
  • 批准号:
    --
  • 项目类别:
    --
  • 资助金额:
    199万元
  • 批准年份:
    2020
  • 负责人:
    刘宝
  • 依托单位: