CONSTRUCTION OF A FRAMEWORK MAP OF CHROMOSOME 18

18 号染色体框架图的构建

• 批准号: 3333647
• 负责人: Thomas Conrad GILLIAM
• 金额: $ 23.52万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-02 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3333647
• 关键词: chromosome disorders; chromosomes; computer assisted sequence analysis; gel electrophoresis; genetic disorder; genetic library; genetic manipulation; genetic markers; genetic polymorphism; genome; human genetic material tag; human population genetics; human tissue; linkage mapping; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; restriction fragment length polymorphism

A strategy is presented to develop a genetic linkage map of chromosome 18. The map will consist of highly polymorphic index markers (heterozygosity > 0.7) spaced not more than 10-15 cM apart and spanning the full estimated genetic length of the chromosome. The linkage map will be composed of new markers developed in our laboratory, including both index and conventional (het < 0.7) markers, and other markers reported by the scientific community. we estimate that an additional 35 index markers are required to complete the project. A strategy is presented to isolate and characterize these markers and to determine their map location and order relative to existing chromosome 18 markers. All new markers will be characterized in the STS format. Through collaboration, new and existing markers will be physically mapped using radiation-reduced cell hybrids, somatic mapping panels, pulse-field gel electrophoresis mapping, and assignment to YAC contigs. Strategies to enrich for index markers indiscriminately across the chromosome as well as strategies to identify markers at discrete loci or chromosomal sub- regions are described. As a priority secondary to development of index markers, we will isolate, characterize, and genetically map less informative markers and begin the construction of a high resolution linkage map. Criteria will be developed to optimize the ultimate utility of the framework map. The optimal relationship between marker heterozygosity and interval spacing will be explored and we will assess the criteria for completion of the genetic map. we will continue to investigate the effects of commonly occurring errors on the genetic linkage map, and corrections for these errors will be incorporated into analysis of the chromosome 18 linkage data. Chromosome 18 resources and information generated as a part of the proposed research will be made immediately available to the research community.

提出了一种染色体遗传连锁图的构建策略 18. 图谱将由高度多态性的索引标记组成 （杂合性> 0.7）间隔不超过10-15 cM， 染色体的完整估计遗传长度。 连锁图 将由我们实验室开发的新标记组成，包括 指标和常规（het < 0.7）标志物，以及其它标志物 据科学界报道。我们估计， 完成项目需要索引标记。 一项战略 提出分离和表征这些标记物，并确定 它们相对于现有的18号染色体标记的图谱位置和顺序。 所有新标记物将以STS格式进行表征。 通过 合作，新的和现有的标记将被物理映射使用 辐射减少的细胞杂交，体细胞定位板，脉冲场凝胶 电泳图谱以及YAC重叠群的分配。 的战略 也在染色体上不加区别地富集索引标记 作为鉴定离散基因座或染色体亚位点上的标记的策略， 区域被描述。 作为一个优先事项，其次是发展指数 标记，我们将隔离，表征和遗传映射较少 信息标记，并开始构建高分辨率 连锁图谱 将制定标准以优化最终效用 的框架图。 标记之间的最佳关系 杂合性和间隔间距将进行探讨，我们将评估 完成基因图谱的标准。我们将继续 研究常见错误对遗传的影响， 连锁图，并纠正这些错误将被纳入 18号染色体连锁数据的分析。 18号染色体资源和 作为拟议研究的一部分产生的信息将被 立即提供给研究界。