CONSORTIUM TO CONSTRUCT CHROMOSOME 3 FRAMEWORK MAP
CONSORTIUM TO CONSTRUCT CHROMOSOME 3 FRAMEWORK MAP
批准号:
3333683
负责人:
SUSAN L NAYLOR
金额:
$15.43万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-04-15 至 1994-03-31
关键词:
chromosome disorders chromosomes cytogenetics gel electrophoresis genetic library genetic manipulation genetic markers genetic polymorphism genome human genetic material tag human population genetics human tissue in situ hybridization linkage mapping molecular cloning nucleic acid probes nucleic acid sequence polymerase chain reaction restriction fragment length polymorphism
中文摘要
3号染色体占人类基因组的7%,约为210
兆。 综合物理和遗传图谱的构建
这条染色体,基于一组共同的DNA标记,将是一个强大的
研究人类遗传学的工具。一个由5个实验室组成的联盟,
3号染色体的优秀资源已经形成了开发10
3号染色体高度多态性标记的cM图谱。迄今为止,
只有有限数量的高度多态性标记开发了这个
染色体 其中四个实验室将分离出高度多态的
来自Cosmos、YAC和流式分选文库的标记,以及
染色体的特定区域的微片段。 多态性将
主要基于(CA)n重复,其将在区域上定位
在物理地图上。 多态性将通过基于PCR的试验进行检测
从(CA)n重复侧翼的序列信息。 此外,
只有轻微或中度多态性的已建立的基因座将被
分析单链构象多态性和梯度凝胶电泳
电泳以增加检测到的多态性水平。 这些
将在CEPH和委内瑞拉家庭中输入试剂,数据将
进行分析以绘制基因图谱。 到两年,我们将产生10个
具有0.7或更大杂合性的标记的cM图谱。 在
第三年,我们将扩大地图,并填补空白,向2-5厘米的地图
这是基因组计划的五年目标。 的发展
人类3号染色体的遗传连锁图将大大促进
生殖系和恶性疾病中疾病基因的鉴定。 一组
这些标记已经被放置在物理和遗传图谱上,
基于序列将大大加快制图进度
3号染色体。
英文摘要
Chromosome 3 has 7% of the human genome and is approximately 210
megabases. The construction of integrated physical and genetic maps for
this chromosome, based on a common set of DNA markers, will be a powerful
tool for studying the genetics of man. A consortium of 5 laboratories all
with excellent resources for chromosome 3 has been formed to develop a 10
cM map of highly polymorphic markers for chromosome 3. To date there have
been only a limited number of highly polymorphic markers developed for this
chromosome. Four of the laboratories will isolate highly polymorphic
markers from cosmids, YACs, and flow sorted libraries as well as
minilibraries for specific regions of the chromosome. Polymorphisms will
be predominantly based on (CA)n repeats which will be regionally localized
on a physical map. The polymorphisms will be detected by a PCR based assay
from sequence information flanking the (CA)n repeats. In addition, well
established loci that are only slightly or moderately polymorphic will be
analyzed for single stranded conformational polymorphisms and gradient gel
electrophoresis to increase the level of polymorphism detected. These
reagents will be typed in CEPH and Venezuelan families and the data will
be analyzed to produce a genetic map. By two years we will generate a 10
cM map of markers that have a heterozygosity of 0.7 or greater. In the
third year we will expand the map and fill in gaps towards the 2-5 cM map
that is the 5 year goal of the Genome Project. The development of a
genetic linkage map of human chromosome 3 will significantly facilitate the
identification of disease genes in germline and malignant disorders. A set
of markers that have been placed on both physical and genetic maps and are
based on sequence will greatly accelerate the progress of mapping
chromosome 3.
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海外基金
小麦部分同源染色体(homoeologous chromosomes)间的定向重组
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批准号:--
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项目类别:--
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资助金额:199万元
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批准年份:2020
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负责人:刘宝
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依托单位: