Nanoparticle Cytometrics: a quantitative analysis of the toxic effect of nanoparticles
纳米颗粒细胞计数:纳米颗粒毒性作用的定量分析
基本信息
- 批准号:EP/H008683/1
- 负责人:
- 金额:$ 117.4万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2009
- 资助国家:英国
- 起止时间:2009 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The last decade has seen an explosion in the development and use of nanoscale technologies and materials and this trend will undoubtedly continue for some time to come. It is clear therefore that human exposure to nanoscale particles will grow dramatically and this has triggered serious concerns over the safety of nanoparticles in relation to human health and the wider ecosystems within which we live. Much of this stems from the paradigm shift incorporated in the move to nano scales which invalidates current chemical risk assessments that are based on bulk properties and hence grossly inappropriate for nano-systems. A marked example of this is provided by noble metals such as Gold; these are traditionally viewed as inert and hence bio-compatible however in nano-particulate form the huge increase in their surface area makes them highly reactive. The issue of surface area has led to the realisation that the quantisation of potential toxins into nano-particulate form is important and that assessment of health risk must take account of specific particle number rather than gross measures of chemical solution based on weight per volume. There is also a growing realisation that the function of particles due to size, shape and chemistry is important. This gap in understanding of chemical hazard at the nanoscale has led to a number of reports from government agencies and royal commissions emphasising the need to develop new measurement techniques to quantify the level of nanoparticle dose and to assess potential toxicity. We propose to use flow cytometry, a laser-based technology, to optically track fluorescent nanoparticles within populations of living cells. This optical measurement approach will be linked to electron microscopy to image particles within cells at the nanoscale. Together these techniques will provide a fully calibrated metrology providing information on number of particles per cell for each and every cell within a measured set (typically a million cells). Our objective is to provide a fundamental understanding of the way in which nanoparticle dose is acquired by cells through natural uptake mechanisms (endocytosis) and then diluted within growing tissue as particles are divided between daughter cells upon cell division (mitosis). A range of cell types will be used to reflect the main exposure routes to nanoparticles, i.e. skin, lung and circulatory (blood) systems. A detailed study of any toxic effects of nanoparticles on the cells will be undertaken and this together with the absolute quantification of dose will allow us to correlate any potential nanotoxicity to the number and form of nanoparticle within a given cell type.
过去十年,纳米技术和材料的开发和使用呈爆炸式增长,这一趋势无疑将持续一段时间。因此,很明显,人类接触纳米级颗粒的数量将急剧增加,这引发了人们对纳米颗粒安全性的严重担忧,这关系到人类健康和我们生活的更广泛的生态系统。这很大程度上源于向纳米尺度转变的范式转变,这使得当前基于整体特性的化学风险评估无效,因此非常不适合纳米系统。一个明显的例子是贵金属,例如黄金;这些传统上被认为是惰性的,因此具有生物相容性,但在纳米颗粒形式中,其表面积的巨大增加使其具有高度反应性。表面积问题使人们认识到,将潜在毒素量化为纳米颗粒形式非常重要,并且健康风险评估必须考虑具体颗粒数量,而不是基于单位体积重量的化学溶液的总测量值。人们也越来越认识到,颗粒的尺寸、形状和化学性质所起的作用非常重要。对纳米级化学危害的理解存在差距,导致政府机构和皇家委员会发布了许多报告,强调需要开发新的测量技术来量化纳米颗粒剂量水平并评估潜在毒性。我们建议使用流式细胞术(一种基于激光的技术)来光学跟踪活细胞群内的荧光纳米颗粒。这种光学测量方法将与电子显微镜相结合,以纳米尺度对细胞内的颗粒进行成像。这些技术共同将提供完全校准的计量学,提供测量组(通常是一百万个细胞)内每个细胞的每个细胞的粒子数量信息。我们的目标是提供对细胞通过自然摄取机制(胞吞作用)获取纳米颗粒剂量的方式的基本了解,然后随着细胞分裂(有丝分裂)时颗粒在子细胞之间分配而在生长的组织中稀释。一系列细胞类型将用于反映纳米颗粒的主要暴露途径,即皮肤、肺和循环(血液)系统。我们将对纳米颗粒对细胞的任何毒性作用进行详细研究,再加上剂量的绝对量化,将使我们能够将任何潜在的纳米毒性与给定细胞类型内纳米颗粒的数量和形式相关联。
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines.
- DOI:10.1016/j.mrgentox.2011.09.013
- 发表时间:2012-06-14
- 期刊:
- 影响因子:1.9
- 作者:Doak, S. H.;Manshian, B.;Jenkins, G. J. S.;Singh, N.
- 通讯作者:Singh, N.
A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia.
- DOI:10.1371/journal.pone.0136382
- 发表时间:2015
- 期刊:
- 影响因子:3.7
- 作者:Corr SJ;Shamsudeen S;Vergara LA;Ho JC;Ware MJ;Keshishian V;Yokoi K;Savage DJ;Meraz IM;Kaluarachchi W;Cisneros BT;Raoof M;Nguyen DT;Zhang Y;Wilson LJ;Summers H;Rees P;Curley SA;Serda RE
- 通讯作者:Serda RE
Quantifying Nanoparticle-Cell Interactions
量化纳米颗粒-细胞相互作用
- DOI:10.1017/s143192761400823x
- 发表时间:2014
- 期刊:
- 影响因子:2.8
- 作者:Hondow N
- 通讯作者:Hondow N
Quantum dot induced cellular perturbations involving varying toxicity pathways
- DOI:10.1039/c4tx00175c
- 发表时间:2015-04
- 期刊:
- 影响因子:2.1
- 作者:Abdullah Al-Ali;Neenu Singh;Bella B. Manshian;T. S. Wilkinson;J. Wills;G. Jenkins;S. Doak
- 通讯作者:Abdullah Al-Ali;Neenu Singh;Bella B. Manshian;T. S. Wilkinson;J. Wills;G. Jenkins;S. Doak
Development of an Optically Transparent Silicon Based Technology Platform for Biological Analysis
开发用于生物分析的光学透明硅基技术平台
- DOI:10.1109/jsen.2014.2367238
- 发表时间:2015
- 期刊:
- 影响因子:4.3
- 作者:Davies N
- 通讯作者:Davies N
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Huw Summers其他文献
Nanoparticles in the life of a cell
细胞生命中的纳米粒子
- DOI:
10.1038/nnano.2011.207 - 发表时间:
2011-11-06 - 期刊:
- 影响因子:34.900
- 作者:
Huw Summers - 通讯作者:
Huw Summers
Huw Summers的其他文献
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{{ truncateString('Huw Summers', 18)}}的其他基金
Engineering Blood Diagnostics: Integrated Platforms for Advanced Detection and Analysis
工程血液诊断:先进检测和分析的集成平台
- 批准号:
EP/N013506/1 - 财政年份:2016
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant
Translational alliance in biophotonics for cytometry
细胞计数生物光子学转化联盟
- 批准号:
EP/N023633/1 - 财政年份:2016
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant
Integrated III-V Haemocytometer
集成 III-V 血细胞计数器
- 批准号:
EP/L005883/1 - 财政年份:2013
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant
Small items of research equipment at Swansea University for an Interdisciplinary Launch-Laboratory
斯旺西大学跨学科发射实验室的小型研究设备
- 批准号:
EP/K031619/1 - 财政年份:2012
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant
Engineering complex fluids in biology and medicine
生物学和医学中的工程复杂流体
- 批准号:
EP/H024271/1 - 财政年份:2010
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant
Stroboscopic excitation fluorescence lifetime imaging
频闪激发荧光寿命成像
- 批准号:
EP/E013104/1 - 财政年份:2007
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant
Towards implanted optoelectronics for in vivo cytomics
用于体内细胞组学的植入光电子学
- 批准号:
G0502180/1 - 财政年份:2006
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant
相似海外基金
Nanoparticle Cytometrics: a quantitative analysis of the toxic effect of nanoparticles
纳米颗粒细胞计数:纳米颗粒毒性作用的定量分析
- 批准号:
EP/H008578/1 - 财政年份:2010
- 资助金额:
$ 117.4万 - 项目类别:
Research Grant