Stroboscopic excitation fluorescence lifetime imaging

频闪激发荧光寿命成像

• 批准号: EP/E013104/1
• 负责人: Huw Summers
• 金额: $ 13.43万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FE013104%2F1
• 关键词: Stroboscopic; excitation; fluorescence; lifetime; imaging

Fluorescence lifetime imaging (FLIM) is a powerful microscopy technique used widely in biology and medicine particularly for imaging cells. It is based on the use of molecules or proteins that fluoresce when illuminated by a light source. The lifetime of the fluorescence is measured rather than its intensity and so the image created gives information on how long the fluorescence lasts for rather than how bright it is. The lifetime is affected by bio-molecular events and so FLIM provides information on the life cycle of the cell as well as images of it.To measure the fluoresence lifetime a fast detector is usually needed as it only lasts for a few billionths of a second. The duration of the measurement is so short that there is a large uncertainty in the measured value. This research proposes a new technique which uses a stream of light pulses to excite the fluoresence. The fluorescence lifetime is then measured by changing the frequency of these pulses. In this case a slow detector can be used and the lifetime measurement done over many seconds thus removing uncertainties in its value. The technique is similar to the use of a stroboscope to allow the human eye ( a slow detector ) to see rapidly moving objects.

荧光寿命成像（FLIM）是一种功能强大的显微技术，广泛应用于生物学和医学，特别是细胞成像。它是基于使用分子或蛋白质，当被光源照射时发出荧光。测量荧光的寿命而不是其强度，因此创建的图像提供了关于荧光持续多长时间而不是它有多亮的信息。寿命受到生物分子事件的影响，因此FLIM提供有关细胞生命周期的信息及其图像。为了测量荧光寿命，通常需要快速检测器，因为它仅持续数十亿分之一秒。测量的持续时间很短，因此测量值存在很大的不确定性。这项研究提出了一种新的技术，使用光脉冲流激发荧光。然后通过改变这些脉冲的频率来测量荧光寿命。在这种情况下，可以使用慢速检测器，并且寿命测量在许多秒内完成，从而消除其值的不确定性。该技术类似于使用频闪观测器，使人眼（一种缓慢的探测器）能够看到快速移动的物体。