THE CONNECTIONS OF THE CINGULATE GYRUS

在一系列关于海马体连接的持续研究中 形成，很明显该结构的输入是 很大程度上是由扣带皮层介导的。 我建议调查 通过使用追踪方法来确定大鼠中后一个区域的连接 顺行和逆行轴浆运输的生理过程。 选择的顺行追踪方法是 3H-氨基酸 放射自显影方法，而逆行追踪将使用 辣根过氧化物酶法和新型荧光染料法 双重标签。 这些数据将与详细的细胞结构进行比较 扣带皮层的分析。 使用放射自显影追踪方法可以检查 该区域的连接不涉及各种光纤系统 位于内侧皮质内或非常靠近内侧皮质。 使用的其他方法将 对于进一步增强我们对这些的详细了解非常有用 连接。 因此，这些研究的结果应该具有直接相关性。 有助于我们了解该区域的电路，更重要的是，它们 应阐明整个边缘系统的组织。

In a continuing series of studies of the connections of the hippocampal formation, it has become apparent that the input of this structues is, to a large extent, mediated by the cingulate cortex. I propose to investigate the connections of this latter area in the rat by using tracing methods which employ the physiological processes of anterograde and retrograde axoplasmic transport. The anterograde tracing method of choice will be the 3H-amino acid autoradiographic method, while the retrograde tracing will be carried out using the horseradish peroxidase method and the new fluorescence dyes method for double labeling. These data will be compared with a detailed cytoarchitectonic analysis of the cingulate cortex. The use of the autoradiographic tracing method will make it possible to examine the connections of this area without involving the various fiber systems which lie within or very close to the mesial cortex. The other methods employed will be extremely useful in further enhancing our detailed knowledge of these connections. Thus, the results of these studies should be of direct relevance to our understanding of the circuitry of this area, and more importantly, they should elucidate the organization of the entire limbic system.