PHYSIOLOGY AND PHARMACOLOGY OF CARDIOVASCULAR NEURONS

这些研究的长期目标是解决 心血管系统的神经调节，特别是心率 和动脉压。电生理和电生理技术的最新进展 解剖学技术提供了检查 参与调节的反射环的中枢神经成分 神经流出到心脏和血管。这里提出的工作是 电生理和免疫细胞化学研究的重点是 内侧动脉压力感受器和其他外周和 中央信息源。第一个目标是检查生理作用 已在孤立的mNTS中识别和表征的六种电流 神经元。这些电流是神经元自发活动的基础， 确定细胞将动作电位传递给 接下来是压力感受器反射通路中的细胞。研究的这一部分将 利用常规微电极电压记录和特定的 通道阻滞剂。然后还将研究这一区域的调制 会影响压力感受器反射通路的活动。其中的两个 物质，谷氨酸和GABA，在同一呼唤下从 突触突触与神经元共同隔离的残留物 传入神经。这是第二个目标的基础，即 继续研究兴奋性和抑制性突触机制 负责自发和刺激的递质释放。 初步证据表明这种释放的突触前调制 也将被追查。第三个目标将探讨 神经元到其他潜在的递质物质，包括 儿茶酚胺、5-羟色胺和P物质。 与其他研究同时进行。这些神经元是 以电气和发射器响应为特征的也将是 使用免疫细胞化学和荧光技术在 存在电势的光和电子显微镜水平 胞体和附着体中的递质物质。结果是 将提供有关压力感受器传输的信息 反射以及是什么调节它，以及提供关于 中枢性突触传递机制。

The long term goal of these studies addresses the mechanisms of the neural regulation of the cardiovascular system, in particular heart rate and arterial pressure. Recent developments in electrophysiological and anatomical techniques have provided the opportunity to examine the central neural components of the reflect loops involved in regulating neural outflow to the heart and blood vessels. The work proposed here is an electrophysiological and immunocytochemical study focusing on the cells of the medial arterial baroreceptors and other peripheral and central sources. The first aim is to examine the physiological role of six currents that have been identified and characterized in isolated mNTS neurons. These currents underlie spontaneous activity in the neuron and determine the ability of the cells to transmit action potentials to the next cells in the baroreflex pathway. This portion of the study will utilized conventional microelectrode voltage recording and specific channel blockers. Modulation of this area will then be studied as too will influence the activity of the baroreflex pathway. Two of these substances, glutamate and GABA, are released on the same call from synaptic boutons that are co-isolated with the neurons as remnants of the afferent innervation. This is the basis of the second aim which continues a study of the excitatory and inhibitory synaptic mechanisms responsible for spontaneous and stimulated transmitter release. Preliminary evidence suggesting presynaptic modulation of this release will also be pursued. The third aim will explore the response of the neurons to other potential transmitter substance including the catecholamines, serotonin and substance P. The fourth aim will be pursued concurrent with the other studies. The neurons that are characterized by electrical and transmitter responses will also be examined using immunocytochemical and fluorescence techniques at the light and electron microscopic level for the presence of potential transmitter substances in the soma and attached boutons. The results will provide information on the transmission across the baroreceptor reflex and what modulates it as well as providing basic information about the mechanism of central synaptic transmission.