METABOLIC PHYSIOLOGY AND SIGNALS OF HEART/LUNG DISEASES

这里提出的调查继续一个程序，以开发和应用新的 组织代谢生理学充分性评价技术 以及对心脏和/或肺部疾病的早期预警， 其他组织，如大脑。 比较将由大脑 线粒体氧化还原功能的“休息”和“活跃”的条件下， 增加对代谢监测的生理基础的理解， 从而，为了增加这些和其它技术的灵敏度， 如磁共振波谱（MRS），作为代谢指标 功能障碍 这些研究将导致努力定义的变化 由威胁代谢完整性的系统性紊乱产生。 的 这项研究的总体假设是，大脑的发病和严重程度 由心肺功能障碍引起的代谢损伤可 通过测量线粒体氧化还原活性的光学技术发出信号。 目标将是开发光学技术作为“警告”指标， 当代谢功能不全受到威胁时， 使用技术作为评估 损伤程度和预后。 光学技术的结果，基于 细胞色素的快速扫描和反射分光光度法， 吡啶核苷酸的荧光测定法将被认为是在可见光和 光谱的红外区域来解释光吸收和组织 散射特性，并提高非侵入性的能力 病人监护 与其他研究者的合作研究 大学将提供一种比较和关联光学数据的方法 从脑电生理学测量中获得的信息， 通过监测代谢功能， 心肺损伤，有可能造成严重损害 大脑和其他脆弱的组织，将有可能定义 全身与组织代谢监测的相对灵敏度 技术，并确定每种技术在信号传导潜力方面的功效， 心脏和/或肺功能障碍威胁组织时的组织损伤 氧合或呼吸。

Investigations proposed here continue a program to develop and apply new technologies for evaluation of the adequacy of tissue metabolic physiology and for early warning of diseases of heart and/or lungs as they impact on other tissues such as brain. Comparisons will be made of brain mitochondrial redox functioning under "resting" and "active" conditions to increase understanding of the physiological basis of metabolic monitoring, and thereby, to increase the sensitivity of these and other techniques, such as magnetic resonance spectroscopy (MRS), as indicators of metabolic dysfunction. These studies will lead to efforts to define the changes produced by systemic derangments that threaten metabolic integrity. The overall hypothesis of this research is that the onset and severity of brain metabolic insults that result from cardiopulmonary dysfunctions can be signalled by optical techniques that measure mitochondrial redox activity. Goals will be to develop optical techniques as "warning" indicators for early intervention when metabolic insufficiency is threatened with the ultimate objective of using techniques as end points for assessment of the extent of injury and for prognosis. Results of optical technologies, based upon rapid-scanning and reflectance spectrophotometry of cytochromes and fluorometry of pyridine nucleotides will be considered in the visible and infrared regions of the spectrum to account for light absorption and tissue scattering properties and to enhance the capability for non-invasive patient monitoring. Collaborative studies with investigators at other universities will provide a means to compare and correlate optical data with information derived from measurements of brain electrophysiology and of tissue metabolites by MRS. By monitoring metabolic functioning during cardiopulmonary insults which have the potential to cause serious damage to the brain and other vulnerable tissues, it will be possible to define the relative sensitivities of systemic vs tissue metabolic monitoring technologies and to determine the efficacy of each in signalling potential tissue injury when heart and/or lung dysfunctions threaten tissue oxygenation or respiration.