METABOLIC PHYSIOLOGY AND SIGNALS OF HEART/LUNG DISEASES

这里提议的调查继续一个项目，以开发和应用新的 评价组织代谢生理学充分性的技术 以及对心脏和/或肺部疾病的早期预警，因为它们影响 其他组织，如脑。我们将对大脑进行比较 线粒体氧化还原在“静息”和“活跃”状态下发挥作用 增加对代谢监测的生理基础的理解， 因此，为了提高这些和其他技术的灵敏度， 例如磁共振波谱(MRS)，作为代谢指标 功能障碍。这些研究将导致努力确定这些变化 由威胁新陈代谢完整性的系统性紊乱所产生。这个 本研究的总体假设是大脑的发病和严重程度 由心肺功能障碍引起的代谢损害可能是 由测量线粒体氧化还原活性的光学技术发出信号。 目标将是开发光学技术作为对 早期干预，当代谢不足威胁到 使用技术作为评估的终点的最终目标 损伤程度和预后。光学技术成果，基于 关于细胞色素和细胞色素的快速扫描和反射光谱 吡啶核苷酸的荧光计量学将考虑在可见光和 用于说明光吸收和组织的红外光谱区域 散射特性和增强非侵入性能力 病人监护。与其他机构的调查人员合作研究 大学将提供一种比较和关联光学数据的方法 通过测量大脑电生理和 通过监测代谢过程中的代谢功能，MRS对组织代谢物的分析 有可能造成严重损害的心肺侮辱 大脑和其他脆弱组织，将有可能定义 全身与组织代谢监测的相对灵敏度 技术，并确定每种技术在信令潜力中的有效性 当心和/或肺功能障碍威胁组织时的组织损伤 氧合或呼吸作用。

Investigations proposed here continue a program to develop and apply new technologies for evaluation of the adequacy of tissue metabolic physiology and for early warning of diseases of heart and/or lungs as they impact on other tissues such as brain. Comparisons will be made of brain mitochondrial redox functioning under "resting" and "active" conditions to increase understanding of the physiological basis of metabolic monitoring, and thereby, to increase the sensitivity of these and other techniques, such as magnetic resonance spectroscopy (MRS), as indicators of metabolic dysfunction. These studies will lead to efforts to define the changes produced by systemic derangments that threaten metabolic integrity. The overall hypothesis of this research is that the onset and severity of brain metabolic insults that result from cardiopulmonary dysfunctions can be signalled by optical techniques that measure mitochondrial redox activity. Goals will be to develop optical techniques as "warning" indicators for early intervention when metabolic insufficiency is threatened with the ultimate objective of using techniques as end points for assessment of the extent of injury and for prognosis. Results of optical technologies, based upon rapid-scanning and reflectance spectrophotometry of cytochromes and fluorometry of pyridine nucleotides will be considered in the visible and infrared regions of the spectrum to account for light absorption and tissue scattering properties and to enhance the capability for non-invasive patient monitoring. Collaborative studies with investigators at other universities will provide a means to compare and correlate optical data with information derived from measurements of brain electrophysiology and of tissue metabolites by MRS. By monitoring metabolic functioning during cardiopulmonary insults which have the potential to cause serious damage to the brain and other vulnerable tissues, it will be possible to define the relative sensitivities of systemic vs tissue metabolic monitoring technologies and to determine the efficacy of each in signalling potential tissue injury when heart and/or lung dysfunctions threaten tissue oxygenation or respiration.