PHYSIOLOGY AND PHARMACOLOGY OF CARDIOVASCULAR NEURONS

本研究开始了对第一突触传递的研究 压力感受器反射 突触位于大脑皮层的一个区域， 髓质由内侧、背内侧和 孤束的背外侧核。 这个区域接受传入神经 压力感受器通路以及许多其他中枢神经系统 系统区域，是心血管整合的主要部位。 重点 该项目将是一个电气和 这一区域神经元的药理学特性。 在这些研究中， 酶促分离细胞。 我们开发了这种制剂 这是第一次有机会研究 参与心血管调节的单个细胞。 膜片钳 全细胞记录技术将用于确定离子 电流负责膜电位，并检查 对假定的递质物质和可乐定等药物的反应 和钙拮抗剂。 这将提供有关 哪些物质参与调节神经活动， 机制使用。 细胞也将被表征， 免疫细胞化学技术。 细胞的属性， 正常血压大鼠（WKY品系）将与自发性高血压大鼠（WKY品系）进行比较。 高血压大鼠（SHR）。 这些研究将作为未来的基础。 研究检查这些压力感受器信息的处理， 二级神经元

This study initiates an investigation of transmission at the first synapse of the baroreceptor reflex. The synapse is located in an area of the medulla which is composed of cells of the medial, dorso-medial and dorso-lateral nuclei of the solitary tract. This area receives afferents from the baroreceptor pathways as well as from many other central nervous system areas and is a major site of cardiovascular integration. The focus of this project will be a characterization of the electrical and pharmacological properties of the neurons in this area. In these studies, the cells are isolated enzymatically. We have developed this preparation which provides, for the first time, the opportunity to study the properties of individual cells involved in cardiovascular regulation. The patch clamp technique for whole cell recording will be used to determine the ionic currents responsible for the membrane potentials and to examine the responses to putative transmitter substances and to such drugs as clonidine and the calcium antagonists. This will provide information concerning which substances are involved in regulating the neural activity and what mechanisms are used. The cells will also be characterized using immunocytochemical techniques. The properties of the cells from normotensive rats (WKY strain) will be compared with those from spontaneous hypertensive rats (SHR). The studies will serve as a foundation for future studies examining the processing of baroreceptor information by these second order neurons.