Beating Hearts at High Resolution: Adaptive High Resolution Selective Plane Illumination Microscopy

高分辨率下的心跳：自适应高分辨率选择性平面照明显微镜

• 批准号: EP/I010173/1
• 负责人: Gordon Love
• 金额: $ 64.54万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FI010173%2F1
• 关键词: Beating; Hearts; Resolution; Adaptive; Selective

In this project we will film life as it develops in the heart by exploiting developments in optical microscopy, high speed imaging, and adaptive optics. The hearts will be in live, developing zebrafish and mouse embryos. This is extremely challenging as the living heart is large (on a microscopic scale), is embedded in the body of the animal (meaning it must be imaged at depth) and is beating, and therefore moving, at a rate of around 300 beats per minute. As well as providing a challenge to demonstrate the advanced methods we will develop the study of cardiac development is important both for investigating the development of the healthy heart and for determining the causes of heart problems.

在这个项目中，我们将利用光学显微镜、高速成像和自适应光学的发展来拍摄生命在心脏中发育的过程。这些心脏将在活的、发育中的斑马鱼和老鼠胚胎中。这是极具挑战性的，因为活着的心脏很大(在显微镜下)，嵌入到动物的身体里(这意味着它必须在深处成像)，正在跳动，因此以每分钟约300次的速度移动。除了提供一个展示先进方法的挑战，我们将发展心脏发育研究对于调查健康心脏的发育和确定心脏问题的原因都是重要的。

项目成果

Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy

宽视场光学显微镜中闭环和无传感器自适应光学器件的比较

• DOI: 10.2971/jeos.2013.13027
• 发表时间: 2013
• 期刊: Rapid Publications
• 作者: Bourgenot C

Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish.

• DOI: 10.1038/s41598-017-05028-2
• 发表时间: 2017-07-11
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Buckley C;Carvalho MT;Young LK;Rider SA;McFadden C;Berlage C;Verdon RF;Taylor JM;Girkin JM;Mullins JJ
• 通讯作者: Mullins JJ

In Smooth Muscle, Mitochondrial Movement is Restricted in Native Cells and Unrestricted and Trafficked When Cells are in Culture

在平滑肌中，线粒体运动在天然细胞中受到限制，而当细胞处于培养状态时则不受限制和运输

• DOI: 10.1016/j.bpj.2009.12.1617
• 发表时间: 2010
• 期刊: Biophysical Journal
• 影响因子: 3.4
• 作者: Chalmers S

3D adaptive optics in a light sheet microscope

• DOI: 10.1364/oe.20.013252
• 发表时间: 2012-06-04
• 期刊: OPTICS EXPRESS
• 影响因子: 3.8
• 作者: Bourgenot, Cyril;Saunter, Christopher D.;Love, Gordon D.
• 通讯作者: Love, Gordon D.

Adaptive optics for wide-field microscopy

用于宽视场显微镜的自适应光学器件

• DOI: 10.1117/12.873857
• 发表时间: 2011
• 作者: Bourgenot C