DIRECT OBSERVATION OF INTERFACIAL PROCESSES--SFM

直接观察界面过程--SFM

• 批准号: 3363349
• 负责人: Joseph Andrade
• 金额: $ 16.94万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3363349
• 关键词: adsorption; biomaterial interface interaction; biomedical equipment development; blood proteins; hydropathy; immunoglobulins; microscopy; polyurethanes; surface property

Nearly all proteins are surface active--they adsorb, orient, and often denature (undergo conformational changes) at water/air, water/oil, and water/solid interfaces. The absorption of contact activation proteins in blood can lead to coagulation; the adsorption of IgG, complement C3 and/or complement C1 can lead to complement activation; the absorption of lipoproteins may be important in atherosclerosis and thrombogenicity. In the case of tears, tear proteins produce deposits on many types of contact lenses. Although there has been much progress in understanding and controlling protein interfacial activity, it is desirable to be able to directly observe protein--surface and protein--protein interactions--in situ--under physiologically relevant conditions. Direct observation is now possible by scanning force microscopy (SFM). This new and very powerful technique is now applicable to protein interface research. We propose to develop the methods, techniques, and theory to permit the routine observation and interpretation of protein interfacial processes. We will apply the technique to three important studies: 1. The orientation and ordering of immunoglobulins at interface. 2. The competitive adsorption of selected plasma proteins--The Vroman Effect. 3. The direct observation of polyurethane surfaces in water.

几乎所有蛋白质都具有表面活性——它们吸附、定向，并且经常 水/空气、水/油和水的变性（经历构象变化） 水/固体界面。 接触激活蛋白的吸收 血液可导致凝固； IgG、补体 C3 和/或的吸附 补体C1可导致补体激活；的吸收 脂蛋白在动脉粥样硬化和血栓形成中可能很重要。 在 就眼泪而言，泪液蛋白会在多种接触方式上产生沉积物 镜头。 尽管在理解和控制方面已经取得了很大进展 蛋白质界面活性，希望能够直接 观察蛋白质--表面和蛋白质--蛋白质相互作用--原位--下 生理相关条件。 现在可以直接观察 扫描力显微镜（SFM）。 这种新的、非常强大的技术是 现在适用于蛋白质界面研究。 我们建议开发方法、技术和理论，以允许 蛋白质界面过程的常规观察和解释。 我们将将该技术应用于三项重要的研究： 1. 界面处免疫球蛋白的方向和排序。 2. 选择性血浆蛋白的竞争吸附--Vroman 影响。 3. 水中聚氨酯表面的直接观察。