REMOTE MONITORING OF PROTEINS INVOLVED IN COAGULATION

远程监测参与凝血的蛋白质

• 批准号: 3352570
• 负责人: Joseph Andrade
• 金额: $ 11.52万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3352570
• 关键词: antibody titering; antithrombin III; antithrombogenic surface; biomaterial interface interaction; cardiovascular prosthesis; clinical biomedical equipment; cow; dyes; extracorporeal circulation; fiber optics; fluorescence spectrometry; heart /lung bypass; hemodialysis; hemostasis; human tissue; immunoglobulins; lasers; monoclonal antibody; patient monitoring device; prothrombin; radiotracer

The blood response to implanted materials and devices is very difficult to monitor. One must either take periodic blood samples (using experienced personnel and very well-defined protocols to minimize artifacts) or use systemically-delivered labelled components to obtain radiographic images, using cine-radiography. The only on-line remote sensors available today include catheter tip blood gas sensors (1) and the light scattering embolus detector (2). Ideally a set of special sensors could monitor the hemostatic situation in animals and patients fitted with in vivo or ex vivo devices, analagous to the pressure and flow sensors in wide use today. We propose to study and to begin the development of specific biochemical sensors which could be used remotely, semicontinuously, and easily for extended periods to provide monitoring of the hemostatic state of patients and of experimental animals with cardio vascular implants. We propose to study a fiber optic sensor based on immobilized monoclonal antibodies (3,4). Specifically we will focus on remote immunosensors to measure antithrombin III and prothrombin. Prothrombin and antithrombin III are selected because of their importance in hemostasis and our experience with these two proteins. The development and study of successful sensors for prothrombin and antithrombin III will provide the experience and technology base for the study of sensors for other components in hemostasis. The research group's long range goal (not proposed herein) is to develop a full hemostatic screen using a multichannel fiber optic device which will provide true multichannel detection, remotely and at least semicontinuously. The availability of such sensors would significantly enhance the study of blood interactions with cardiovascular and related devices. This proposal is a resubmission. The original application was criticized as too broad and diffuse. This application is focused on two available and important coagulation proteins which provide a practical model system for studying the remote sensor concepts.

血液对植入材料和装置的反应很难确定 监视器。 必须定期采集血液样本（使用有经验的 人员和非常明确的协议，以最大限度地减少工件）或使用 系统地传递标记成分以获得放射线图像， 使用电影放射线摄影术。 当今唯一可用的在线远程传感器 包括导管尖端血气传感器 (1) 和光散射栓塞 探测器 (2)。 理想情况下，一组特殊传感器可以监控 动物和患者体内或离体的止血情况 设备，类似于当今广泛使用的压力和流量传感器。 我们建议研究并开始开发特定的生化药物 可以远程、半连续且轻松地使用的传感器 延长监测患者止血状态的时间 以及植入心血管的实验动物。 我们建议研究一种基于固定化单克隆抗体的光纤传感器 抗体(3,4)。 具体来说，我们将重点关注远程免疫传感器 测量抗凝血酶 III 和凝血酶原。 凝血酶原和抗凝血酶 III 选择它们是因为它们在止血方面的重要性以及我们的经验 与这两种蛋白质。 成功传感器的开发和研究 对于凝血酶原和抗凝血酶 III 将提供经验和 其他止血元件传感器研究的技术基础。 该研究小组的长期目标（本文未提出）是开发一种 使用多通道光纤装置的全止血屏幕，该装置将 提供真正的多通道检测，远程且至少 半连续地。 此类传感器的可用性将显着 加强血液与心血管及相关疾病相互作用的研究 设备。 该提案是重新提交。 最初的申请受到批评 过于宽泛和分散。 该应用程序专注于两个可用的和 重要的凝血蛋白，提供了实用的模型系统 研究遥感器概念。