REMOTE MONITORING OF PROTEINS INVOLVED IN COAGULATION

血液对植入材料和装置的反应很难 监视器。必须定期采集血样(使用有经验的 人员和非常明确的协议以最大限度地减少人工制品)或使用 系统地递送标记的组件以获得射线照相图像， 使用电影射线照相术。目前唯一可用的在线遥控器 包括导管尖端血气传感器(1)和光散射栓子 检测器(2)。理想情况下，一组特殊的传感器可以监控 动物和患者体内和体外的止血情况 设备，类似于今天广泛使用的压力和流量传感器。 我们建议研究并开始开发特定的生物化学 传感器可远程、半连续、轻松地用于 延长监测病人止血状态的时间 以及心脏血管植入物的实验动物。 我们提出研究一种基于固定化单抗的光纤传感器 抗体(3，4)。具体地说，我们将重点介绍远程免疫传感器 测定抗凝血酶III和凝血酶原。凝血酶原和抗凝血酶III 之所以被选中是因为它们在止血方面的重要性和我们的经验 这两种蛋白质的结合。成功传感器的开发与研究 对于凝血酶原和抗凝血酶III将提供经验和 为止血中其他成分传感器的研究奠定了技术基础。 研究小组的长期目标(这里没有提出)是开发一种 使用多通道光纤设备的全止血屏幕，将 远程提供真正的多通道检测，至少 半连续地。这种传感器的可用性将显著地 加强血液与心血管及相关疾病相互作用的研究 设备。 这项提案是重新提交的。最初的申请受到了批评 因为过于宽泛和分散。此应用程序侧重于两个可用的和 重要的凝血蛋白，提供了一个实用的模型系统 学习遥感器的概念。

The blood response to implanted materials and devices is very difficult to monitor. One must either take periodic blood samples (using experienced personnel and very well-defined protocols to minimize artifacts) or use systemically-delivered labelled components to obtain radiographic images, using cine-radiography. The only on-line remote sensors available today include catheter tip blood gas sensors (1) and the light scattering embolus detector (2). Ideally a set of special sensors could monitor the hemostatic situation in animals and patients fitted with in vivo or ex vivo devices, analagous to the pressure and flow sensors in wide use today. We propose to study and to begin the development of specific biochemical sensors which could be used remotely, semicontinuously, and easily for extended periods to provide monitoring of the hemostatic state of patients and of experimental animals with cardio vascular implants. We propose to study a fiber optic sensor based on immobilized monoclonal antibodies (3,4). Specifically we will focus on remote immunosensors to measure antithrombin III and prothrombin. Prothrombin and antithrombin III are selected because of their importance in hemostasis and our experience with these two proteins. The development and study of successful sensors for prothrombin and antithrombin III will provide the experience and technology base for the study of sensors for other components in hemostasis. The research group's long range goal (not proposed herein) is to develop a full hemostatic screen using a multichannel fiber optic device which will provide true multichannel detection, remotely and at least semicontinuously. The availability of such sensors would significantly enhance the study of blood interactions with cardiovascular and related devices. This proposal is a resubmission. The original application was criticized as too broad and diffuse. This application is focused on two available and important coagulation proteins which provide a practical model system for studying the remote sensor concepts.