REMOTE MONITORING OF PROTEINS INVOLVED IN COAGULATION

血液对植入材料和器械的反应很难确定。 监视器. 必须定期采集血液样本（使用经验丰富的 人员和非常明确的协议，以最大限度地减少伪影）或使用 系统递送的标记的组分以获得射线照相图像， 使用电影射线照相术。 今天唯一可用的在线遥感器 包括导管尖端血气传感器（1）和光散射栓子， 检测器（2）。 理想情况下，一组特殊的传感器可以监测 体内或离体装配的动物和患者的止血情况 这种传感器类似于当今广泛使用的压力和流量传感器。 我们建议研究并开始开发特定的生化 传感器，可以远程使用，连续，很容易， 延长时间以监测患者的止血状态 以及植入心脏血管的实验动物。 我们提出研究一种基于固定化单克隆抗体的光纤传感器 抗体（3，4）。 具体来说，我们将重点关注远程免疫传感器， 测量抗凝血酶III和凝血酶原。 凝血酶原和抗凝血酶III 由于其在止血方面的重要性和我们的经验， 这两种蛋白质。 成功的传感器的开发和研究 凝血酶原和抗凝血酶III将提供经验， 止血中其他组件传感器研究的技术基础。 该研究小组的长期目标（本文未提出）是开发一种 使用多通道光纤装置进行全面止血筛选， 提供真正的多通道检测，远程和至少 不间断地。 这种传感器的可用性将大大 加强血液与心血管和相关疾病相互作用的研究 装置. 这个建议是重新提出的。 最初的申请受到了批评 过于宽泛和分散。 该应用程序集中在两个可用的， 重要的凝血蛋白质，提供了一个实用的模型系统， 研究遥感器的概念。

The blood response to implanted materials and devices is very difficult to monitor. One must either take periodic blood samples (using experienced personnel and very well-defined protocols to minimize artifacts) or use systemically-delivered labelled components to obtain radiographic images, using cine-radiography. The only on-line remote sensors available today include catheter tip blood gas sensors (1) and the light scattering embolus detector (2). Ideally a set of special sensors could monitor the hemostatic situation in animals and patients fitted with in vivo or ex vivo devices, analagous to the pressure and flow sensors in wide use today. We propose to study and to begin the development of specific biochemical sensors which could be used remotely, semicontinuously, and easily for extended periods to provide monitoring of the hemostatic state of patients and of experimental animals with cardio vascular implants. We propose to study a fiber optic sensor based on immobilized monoclonal antibodies (3,4). Specifically we will focus on remote immunosensors to measure antithrombin III and prothrombin. Prothrombin and antithrombin III are selected because of their importance in hemostasis and our experience with these two proteins. The development and study of successful sensors for prothrombin and antithrombin III will provide the experience and technology base for the study of sensors for other components in hemostasis. The research group's long range goal (not proposed herein) is to develop a full hemostatic screen using a multichannel fiber optic device which will provide true multichannel detection, remotely and at least semicontinuously. The availability of such sensors would significantly enhance the study of blood interactions with cardiovascular and related devices. This proposal is a resubmission. The original application was criticized as too broad and diffuse. This application is focused on two available and important coagulation proteins which provide a practical model system for studying the remote sensor concepts.