MULTI-ANALYTE MICRO-DEVICES FOR BIOMEDICAL APPLICATIONS

描述（由申请人提供）：研究者建议开发用于小样本量生物液体中多种代谢物的特定化学分析的微型装置。 特异性和灵敏度由特异性反应提供，所述特异性反应将分析物偶联到基于生物发光的酶反应并产生与分析物浓度成比例的光。 生物发光分析测定以小型化和稳定的形式，可以测量微升样品体积中的亚微摩尔浓度。 第一阶段（R21）的目标是设计微流控结构，开发酶包装和稳定技术，并优化光学检测系统，以便使用生物发光反应测量两种模型分析物溶液（半乳糖和乳酸盐）。 第二阶段（R33）的目标是在微流控检测系统中实施其他生物发光检测，开发特定的诊断面板，利用实际的生物流体样品，并提高分析的准确性和精密度。 拟议的微量分析系统（microAS）将便于在护理点（POC）和家庭环境中操作。 它可能会发展到从一个1-100微升的生物流体样本中测量亚微摩尔到毫摩尔范围内的多达100种不同代谢物，并包括用于基础研究，临床研究以及个人疾病和健康管理的定制综合诊断面板。 这些系统将提供快速的结果，促进患者赋权，并降低医疗保健成本。

DESCRIPTION (provided by applicant): The investigators propose to develop microdevices for the specific chemical analysis of multiple metabolites in small sample volumes of biological fluids. The specificity and sensitivity is provided by specific reactions that couple analytes to bioluminescent-based enzyme reactions and produce light proportional to the analyte concentration. Bioluminescent analytical assays, in a miniaturized and stable format, can measure sub-microMolar concentrations in microliter sample volumes. The goal of the first phase (R21) is to engineer microfluidic structures, develop enzyme packaging and stabilization techniques, and optimize optical detection systems in order to measure two model analyte solutions (galactose and lactate) using bioluminescent reactions. The goal of the second phase (R33) is to implement other bioluminescent assays in the microfluidic detection system, develop specific diagnostic panels, utilize practical biofluid samples and enhance analytical accuracy and precision. The proposed Micro-Analytical System (microAS) will be convenient to operate in point-of-care (POC) and home environments. It will likely evolve to measure up to 100 different metabolites in the submicroMolar to milliMolar range from one 1-100 microL biofluid sample, and include customized comprehensive diagnostic panels for basic research, clinical research, and for personal disease and health management. These systems would provide rapid results, facilitate patient empowerment, and reduce health care costs.