DIRECT OBSERVATION OF INTERFACIAL PROCESSES--SFM

直接观察界面过程--SFM

• 批准号: 3363351
• 负责人: Joseph Andrade
• 金额: $ 14.19万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3363351
• 关键词: adsorption; biomaterial interface interaction; biomedical equipment development; blood proteins; hydropathy; immunoglobulins; microscopy; polyurethanes; surface property

Nearly all proteins are surface active--they adsorb, orient, and often denature (undergo conformational changes) at water/air, water/oil, and water/solid interfaces. The absorption of contact activation proteins in blood can lead to coagulation; the adsorption of IgG, complement C3 and/or complement C1 can lead to complement activation; the absorption of lipoproteins may be important in atherosclerosis and thrombogenicity. In the case of tears, tear proteins produce deposits on many types of contact lenses. Although there has been much progress in understanding and controlling protein interfacial activity, it is desirable to be able to directly observe protein--surface and protein--protein interactions--in situ--under physiologically relevant conditions. Direct observation is now possible by scanning force microscopy (SFM). This new and very powerful technique is now applicable to protein interface research. We propose to develop the methods, techniques, and theory to permit the routine observation and interpretation of protein interfacial processes. We will apply the technique to three important studies: 1. The orientation and ordering of immunoglobulins at interface. 2. The competitive adsorption of selected plasma proteins--The Vroman Effect. 3. The direct observation of polyurethane surfaces in water.

几乎所有蛋白质都表面活跃 - 它们吸附，东方和通常 在水/空气，水/石油和 水/实心界面。 接触激活蛋白在 血液会导致凝血； IgG，补充C3和/或的吸附 补体C1可以导致补体激活；吸收 脂蛋白在动脉粥样硬化和血栓形成性中可能很重要。 在 眼泪的情况下，泪液蛋白质在许多类型的接触上产生沉积物 镜片。 尽管在理解和控制方面取得了很多进展 蛋白质界面活性，希望能够直接 观察蛋白质 - 表面和蛋白质 - 蛋白质相互作用 - 原位 - 生理上相关的条件。 现在可以直接观察 扫描力显微镜（SFM）。 这种新的非常强大的技术是 现在适用于蛋白质界面研究。 我们建议开发方法，技术和理论，以允许 蛋白质界面过程的常规观察和解释。 我们将将该技术应用于三个重要研究： 1。接口处免疫球蛋白的方向和顺序。 2。选定的血浆蛋白的竞争吸附 - vroman 影响。 3。直接观察水中聚氨酯表面。