Multi-Technique Bio-Analytical Investigation at the Single / Sub-Cellular Level Using a New Lab-On-A-Chip Technology Platform

使用新的芯片实验室技术平台在单/亚细胞水平上进行多技术生物分析研究

• 批准号: EP/I038799/1
• 负责人: Paul Holland
• 金额: $ 12.47万
• 依托单位: Swansea University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FI038799%2F1
• 关键词: Multi; Technique; Bio; Analytical; Investigation

Advances in the 'silicon chip' industry during the 20th century led to the development of new microchip processing technologies that have allowed the continued miniaturisation of electronic and mechanical devices. These advances were adopted by and contributed to 'on-chip' tools to enable the massive boost in knowledge and invention within the Biotechnology area. A Biochip is a polymer, glass or silicon chip with integrated devices and features that can be used to better understand biochemical processes that occur in human beings. This new knowledge and information can be then used to develop new or better treatments for diseases. More recently there has been a research drive to integrate 'on-chip' all the necessary sensors, micro-fluidics and analysis tools required for specific biological experiments, in this case the chip can be considered as a self contained laboratory. This technology is called Lab-On-A-Chip (LOAC) and has tremendous scope to achieve further advances in knowledge across differing scientific disciplines such as health, technology and surface science. The biotechnology industry has made highly significant contributions to health and the global economy with the invention of the DNA microarray for example. Research groups working in the LOAC area are focusing significant research effort into various technologies that allow biological investigation at the single cell or sub-cellular level. These chips require the ability to sort cells by type, separate them and deliver them in a controlled manner to an experimental micro-site for experimentation. New tools are now becoming commercially available that can analyse the cells at the micro-site simultaneously combining 'top down' AFM force measurement and imaging with 'bottom up' advanced optical investigation. Where combined AFM and optical analytical techniques have been used the LOAC technologies used have been required to be glass based due to the required transparent characteristic. This has prevented the use of silicon chip based LOAC technology that would allow the development of new sensor functions and for example the dielectrophoretic control of cells to be performed 'on-chip' reducing size and cost.This research program aims to design, fabricate and test a novel silicon Lab-On-A-Chip (LOAC) structure with optical transparent characteristics that allow multi-technique singular or sub-cell investigation and analysis using AFM and epi-fluorescence microscopy. The use of a Complementary Metal Oxide Semiconductor (CMOS) compatible process to fabricate the new LOAC structure enables scope for future 'on chip' integration of analytical techniques combined with the processing power of integrated silicon chip technology. This research will provide a novel technology platform that can be developed with future innovations leading to new tools and thereby better understanding of the immunological response that underpins many disease states including allergy, diabetes and cancer.

在20世纪，“硅芯片”行业的进步导致了新的微芯片加工技术的发展，这些技术使电子和机械设备的持续微型化。这些进步是通过“片上”工具采用并促进了生物技术领域内知识和发明的大量提高的并做出了贡献的原因。生物芯片是具有集成设备和特征的聚合物，玻璃或硅芯片，可用于更好地理解人类中发生的生化过程。然后，这些新知识和信息可用于开发新的或更好的疾病治疗方法。最近，已经进行了研究驱动力，以整合“片上”所有必要的传感器，特定生物学实验所需的微富集化学和分析工具，在这种情况下，可以将芯片视为自身包含的实验室。这项技术称为实验室芯片（LOAC），具有巨大的范围，可以在诸如健康，技术和表面科学等不同科学学科的知识方面取得进一步的进步。例如，通过DNA微阵列的发明，生物技术行业为健康和全球经济做出了极大的贡献。在LOAC地区工作的研究小组正在将大量的研究工作集中在各种技术上，这些技术允许在单细胞或细胞级上进行生物学研究。这些芯片需要能够按类型对细胞进行分类，将它们分开并以受控方式将其交付到实验微位置进行实验。现在，新工具正在商业上可用，可以同时将“自上而下”的AFM力测量和成像与“自下而上”的高级光学研究相结合的微位置分析细胞。如果使用了合并的AFM和光学分析技术，则由于所需的透明特性，使用的LOAC技术是基于玻璃的。 This has prevented the use of silicon chip based LOAC technology that would allow the development of new sensor functions and for example the dielectrophoretic control of cells to be performed 'on-chip' reducing size and cost.This research program aims to design, fabricate and test a novel silicon Lab-On-A-Chip (LOAC) structure with optical transparent characteristics that allow multi-technique singular or sub-cell investigation and analysis using AFM和荧光显微镜。使用互补的金属氧化物半导体（CMOS）兼容过程来制造新的LOAC结构，可实现未来的“芯片”整合分析技术的整合，并结合了集成硅芯片技术的处理能力。这项研究将提供一个新颖的技术平台，可以通过未来的创新进行开发，从而为新工具提供了新的工具，从而更好地了解了基于许多疾病国家（包括过敏，糖尿病和癌症）的免疫学反应。

项目成果

An Optically Transparent Silicon CMOS Lab-On-A-Chip Technology Platform for Single Cell Analysis

用于单细胞分析的光学透明硅 CMOS 片上实验室技术平台

• 作者: Paul Holland (Speaker)

A New CMOS Compatible Lab-On-A-Chip Platform with Through-Wafer Optical Transparency for Advanced Biological Investigation

新型 CMOS 兼容片上实验室平台，具有晶圆间光学透明度，适用于高级生物研究

• 作者: Paul Holland (Co-Author)

Development of an Optically Transparent Silicon Based Technology Platform for Biological Analysis

开发用于生物分析的光学透明硅基技术平台

• DOI: 10.1109/jsen.2014.2367238
• 发表时间: 2015
• 期刊: IEEE Sensors Journal
• 影响因子: 4.3
• 作者: Davies N